PXD020509

PXD020509 is an original dataset announced via ProteomeXchange.

Title	PASEF technology on timsTOF Pro improves quality of FPOP analysis
Description	Fast photochemical oxidation of proteins (FPOP) is a modern technique for studying protein folding, conformations, interactions, etc. In this work, timsTOF Pro mass spectrometer coupled with trapped ion mobility spectrometry was used for identification of oxidative modifications in a model protein. Multiple modifications on the same residues, including six modifications of histidine, were successfully resolved. Moreover, PASEF technology allows successful sequencing even minor populations of modified peptides.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-13_16:51:41.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dmitry Loginov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-22 08:17:58	ID requested
1	2021-06-10 01:05:59	announced
⏵ 2	2022-02-13 16:51:42	announced	2022-02-14: Updated project metadata.

Loginov DS, Fiala J, Chmelik J, Brechlin P, Kruppa G, Novak P, Benefits of Ion Mobility Separation and Parallel Accumulation-Serial Fragmentation Technology on timsTOF Pro for the Needs of Fast Photochemical Oxidation of Protein Analysis. ACS Omega, 6(15):10352-10361(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: TIMS, PASEF,timsTOF, protein footprinting, hydroxyl radical, FPOP

Petr Novak
contact affiliation	Institute of Microbiology of the CAS (BioCeV)
contact email	pnovak@biomed.cas.cz
lab head
Dmitry Loginov
contact affiliation	BioCeV - Institute of Microbiology of the CAS
contact email	dloginov@jcu.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD020509

PRIDE project URI

• PRIDE
  1. PXD020509
     1. Label: PRIDE project
     2. Name: PASEF technology on timsTOF Pro improves quality of FPOP analysis