PXD020503

PXD020503 is an original dataset announced via ProteomeXchange.

Title	Secretome Analysis of hippocampal and cortical murine neurons
Description	To understand how cells communicate with each other, it is essential to define the cellular secretome, a collection of proteins including soluble secreted, unconventionally secreted and proteolytically-shed proteins. Quantitative methodologies to decipher the secretome are challenging, due to the requirement of large cell numbers and abundant serum proteins that interfere with the detection of low-abundant cellular secretome proteins. Here, we have use the highe perfomance secretome-protein-enrichment-with-click-sugars method (hiSPECS) for glyco-secretome analysis. We applied this method to investigate differences of hippocampal and cortical murine neurons. Additionally, we have inhibited the Alzheimer related protease BACE1 to identify potential substrates in the secretome of hippocampal neurons.
HostingRepository	PRIDE
AnnounceDate	2020-09-25
AnnouncementXML	Submission_2020-09-25_03:28:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-22 07:53:13	ID requested
⏵ 1	2020-09-25 03:28:13	announced

T, ü, shaus J, M, ü, ller SA, Kataka ES, Zaucha J, Sebastian Monasor L, Su M, G, ü, ner G, Jocher G, Tahirovic S, Frishman D, Simons M, Lichtenthaler SF, An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome. EMBO J, 39(20):e105693(2020) [pubmed]

submitter keyword: secretome, BACE1, neurons

Stefan F. Lichtenthaler
contact affiliation	Neuroproteomics DZNE Munich Feodor-Lynen-Str. 17 81377 Munich Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020503

PRIDE project URI

• PRIDE
  1. PXD020503
     1. Label: PRIDE project
     2. Name: Secretome Analysis of hippocampal and cortical murine neurons