PXD020502

PXD020502 is an original dataset announced via ProteomeXchange.

Title	VAMP7 KO and ATG5 KO in axonal growth
Description	VAMP7 is involved in autophagy and in exocytosis mediating neurite growth, two yet unconnected cellular pathways. Here we found that nutrient restriction and activation of autophagy stimulated axonal growth while inhibition led to multiple axons. VAMP7 KO neuronal cells showed impaired whereas ATG5 KO cells showed increased neurite growth. Secretomics identified that ER-phagy-related LC3 interacting region-containing proteins Atlastins and Reticulons were more abundant in ATG5 KO and less abundant in VAMP7 KO secretomes. The secretion of reticulon 3, LC3-II and P62 was impaired in VAMP7 KO cells. Treatment of neuronal cells with ATG5 or VAMP7 KO conditioned medium showed no effect thus the role of ATG5 and VAMP7 in neurite growth was cell autonomous. A nanobody directed against VAMP7 inhibited axonal growth induced by nutrient restriction. Furthermore, expression of the inhibitory Longin domain of VAMP7 impaired the subcellular localization of reticulon 3 in neurons. We propose that VAMP7-dependent unconventional autophagy-dependent secretion is an essential mechanism for axonal growth.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:35.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-22 07:45:00	ID requested
1	2021-03-29 10:09:28	announced
⏵ 2	2024-10-22 05:20:36	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: secretion, neurons,VAMP7, amphisomes, axonal growth

Chiara Guerrera
contact affiliation	Proteomics platform Necker, Université de Paris - Structure Fédérative de Recherche Necker, INSERM US24/CNRS UMS3633, Paris 75015, France.
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD020502

PRIDE project URI

• PRIDE
  1. PXD020502
     1. Label: PRIDE project
     2. Name: VAMP7 KO and ATG5 KO in axonal growth