PXD020471

PXD020471 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison of HliD and Ycf39 protein abundances in FLAG-tagged chlorophyll synthase co-immunoprecipitation assays of control and CrtR/CruF (carotenoid biosynthesis) knockout strains of Synechocystis sp. PCC 6803.
Description	Chlorophyll a synthase (ChlG) is the enzyme responsible for attaching either a geranygeranyl or phytyl group to the chlorophyllide macrocycle during chlorophyll biosynthesis. Previous co-immunoprecipitation assays using FLAG-tagged ChlG identified the high light inducible protein HliD and the photosystem II assembly factor Ycf39 among the interaction partners. Non-protein components of this complex are the carotenoids zeaxanthin and myxoxanthophyll. To elucidate the function of these carotenoids in the association of ChlG/HliD/Ycf39, knockout strains of Synechocystis were produced to disrupt carotenoid biosynthesis. The abundances of HliD and Ycf39 relative to FLAG-tagged ChlG were compared in the different strains by proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:35.827.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationList	methylthiolated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-21 06:12:35	ID requested
1	2022-08-18 06:03:36	announced
⏵ 2	2023-11-14 08:51:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: zeaxanthin, chlorophyll a synthase, FLAG-tag, HliD, myxoxanthophyll,Synechocystis sp. PCC 6803, Ycf39, CrtR, CruF.

Andrew Hitchcock
contact affiliation	Department of Molecular Biology and Biotechnology, The University of Sheffield, UK.
contact email	a.hitchcock@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020471
     1. Label: PRIDE project
     2. Name: Proteomic comparison of HliD and Ycf39 protein abundances in FLAG-tagged chlorophyll synthase co-immunoprecipitation assays of control and CrtR/CruF (carotenoid biosynthesis) knockout strains of Synechocystis sp. PCC 6803.