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PXD020462

PXD020462 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOxidative damage signatures in human bone collagen from Pompeii and Herculaneum.
DescriptionIn search of markers for protein aging, we used an extraordinary test case of human bones from the excavations sites of Pompei and Herculaneum (79 AD), analyzed by a shotgun proteomic approach using LC-MS/MS. Human bones from Scalandrone Bay (II sec AD, another volcanic area, yet not related to Vesuvius eruption were also analysed for the comparison of the PTMs provoked from a volcanic ground to an almost coeval standard burial condition. In addition, already published archaeological bones, approximately 17 centuries younger than the test case, were used as control samples. Deamidation of asparagine and glutamine was examined as a now widely considered aging molecular marker. Deamidation profile was quite robust for samples with a similar age whereas the fewer proteins were detected, the more degraded the sample were found to be. Positional investigation of deamidation in the polypeptide sequence of collagen type I revealed that some zones are more susceptible to deamidation than others. Back bone cleavage analysis confirmed that some zones apart from deamidation are more susceptible to spontaneous hydrolysis too. Analysis of post-translational modifications showed higher advanced glycation products of lysine and arginines in the samples of Pompeii and Herculaneum. Furthermore, oxidative products of methionine, histidine and the conversion of ST to glycine were also detected. Interestingly, multiple oxidation products of proline and dehydration of hydroxyproline were detected for the first time, turning the scientific interest to this amino acid (P), which is usually ignored due to its natural conversion to hydroxyproline. As a matter of fact, we can observe that collagen degradation in bones from Pompeii and Herculaneum underwent different degradation processes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:23:53.422.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGeorgia Ntasi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdihydroxylated residue; L-ornithine (Arg); N6-carboxymethyl-L-lysine; Oxohistidine (from histidine); dehydrated residue; N6-1-carboxyethyl-L-lysine; methylglyoxal arginine adduct (+54 amu); formylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-20 22:11:58ID requested
12021-07-10 03:56:11announced
22024-10-22 05:23:53announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: paleoproteomics,Human bones, Pompeii, AGEs,Deamidation (NQ),Vesuvius,79 AD Herculaneum, Shotgun proteomics
Contact List
Leila Birolo
contact affiliationDepartment of Chemical Sciences, University of Federico II,Naples, Italy
contact emailbirolo@unina.it
lab head
Georgia Ntasi
contact affiliationUniversity of Naples "Federico II"
contact emailgeorgiant91@gmail.com
dataset submitter
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Dataset FTP location
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