PXD020457

PXD020457 is an original dataset announced via ProteomeXchange.

Title	Interactome of Irgm2 in mouse macrophages
Description	This dataset corresponds to several experiments performed in order to identify proteins interacting with Immunity-related GTPase family M member 2 (Irgm2) in murine macrophages. To that aim, a version of the protein fused with the GFP (Egfp-Irgm2) was transduced in immortalized murine Bone Marrow-Derived Macrophages (iBMDMs), and Irgm2 complexes were subsequently purified using anti-GFP Trap technology. Different experiments were performed using either cells initially primed by treatment with interferon gamme (IFNg) or without any priming (NP), and in each case, cells were either infected with Salmonella (STm) or non infected (mock), leading to 4 different conditions. Corresponding control samples were prepared in the same way using macrophages transduced with GFP only (Egfp), leading to 8 different types of samples. Three independent biological experiments were performed for each condition, and each sample was analysed in triplicate by mass spectrometry, resulting in 72 LC-MS runs contained in the dataset.
HostingRepository	PRIDE
AnnounceDate	2020-11-03
AnnouncementXML	Submission_2020-11-03_04:12:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-20 09:34:56	ID requested
⏵ 1	2020-11-03 04:12:45	announced

Dataset with its publication pending

submitter keyword: Irgm2, interactome, macrophage, primary T cell, AP-MS

Odile Schiltz
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse
contact email	Odile.Schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD020457

PRIDE project URI

• PRIDE
  1. PXD020457
     1. Label: PRIDE project
     2. Name: Interactome of Irgm2 in mouse macrophages