PXD020449

PXD020449 is an original dataset announced via ProteomeXchange.

Title	Interactomes of Macrophage migration inhibitory factor (MIF) and its homologue D-dopachrome tautomerase (D-DT )
Description	NIH 3T3 fibroblasts that express little detectable levels of the MIF binding receptor CD74 but do express the signalling component CD44 were used for the identification of interacting proteins that are shared by MIF and D-DT. Endogenously expressed MIF and D-DT were tagged at the C-terminus using a short sequence that is recognized and biotinylated by the bacterial biotin ligase BirA (Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. Proc. Natl. Acad. Sci. U. S. A. 100, 7480–5) as described in detail for MIF Filip, A. M. et al. (2009). Ribosomal Protein S19 Interacts with Macrophage Migration Inhibitory Factor and Attenuates Its Pro-inflammatory Function. J. Biol. Chem. 284, 7977–7985). After pulling down biotinylated proteins from stable NIH 3T3 clones that express tagged MIF or D-DT and BirA ligase with streptavidin agarose, bound proteins were separated by SDS-PAGE and identified after elution and tryptic digestion by mass spectrometry in order to characterize the MIF and D-DT interactomes.
HostingRepository	PRIDE
AnnounceDate	2021-01-27
AnnouncementXML	Submission_2021-01-27_13:48:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020449
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jörg Klug
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q-Tof Ultima; LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-20 04:26:18	ID requested
⏵ 1	2021-01-27 13:48:24	announced

Li JH, Tang Y, Lv J, Wang XH, Yang H, Tang PMK, Huang XR, He ZJ, Zhou ZJ, Huang QY, Klug J, Meinhardt A, Fingerle-Rowson G, Xu AP, Zheng ZH, Lan HY, Macrophage migration inhibitory factor promotes renal injury induced by ischemic reperfusion. J Cell Mol Med, 23(6):3867-3877(2019) [pubmed]

Li J, Tang Y, Tang PMK, Lv J, Huang XR, Carlsson-Skwirut C, Da Costa L, Aspesi A, Fr, ö, hlich S, Szcz, ę, ś, niak P, Lacher P, Klug J, Meinhardt A, Fingerle-Rowson G, Gong R, Zheng Z, Xu A, Lan HY, Blocking Macrophage Migration Inhibitory Factor Protects Against Cisplatin-Induced Acute Kidney Injury in Mice. Mol Ther, 26(10):2523-2532(2018) [pubmed]

Filip AM, Klug J, Cayli S, Fr, ö, hlich S, Henke T, Lacher P, Eickhoff R, Bulau P, Linder M, Carlsson-Skwirut C, Leng L, Bucala R, Kraemer S, Bernhagen J, Meinhardt A, Ribosomal protein S19 interacts with macrophage migration inhibitory factor and attenuates its pro-inflammatory function. J Biol Chem, 284(12):7977-85(2009) [pubmed]

10.1242/jcs.217356;

Cayli S, Klug J, Chapiro J, Fr, ö, hlich S, Krasteva G, Orel L, Meinhardt A, COP9 signalosome interacts ATP-dependently with p97/valosin-containing protein (VCP) and controls the ubiquitination status of proteins bound to p97/VCP. J Biol Chem, 284(50):34944-53(2009) [pubmed]

submitter keyword: Interactome, MIF, D-DT, NIH 3T3

Prof. Dr. Andreas Meinhardt
contact affiliation	Justus Liebig University Giessen Department of Anatomy and Cell Biology Aulweg 123 35392 Giessen
contact email	andreas.meinhardt@anatomie.med.uni-giessen.de
lab head
Jörg Klug
contact affiliation	Justus Liebig University Institut für Anatomie und Zellbiologie Aulweg 123 35392 Giessen
contact email	joerg.klug@anatomie.med.uni-giessen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD020449

PRIDE project URI

• PRIDE
  1. PXD020449
     1. Label: PRIDE project
     2. Name: Interactomes of Macrophage migration inhibitory factor (MIF) and its homologue D-dopachrome tautomerase (D-DT )