PXD020436

PXD020436 is an original dataset announced via ProteomeXchange.

Title	Global proteomic analysis of extracellular matrix in mouse and human brain highlights association with cerebrovascular disease
Description	The extracellular matrix (ECM), a key interface between the cerebrovasculature and other cells within the neuro-glial-vascular unit , provides structural stability and modulates cell behaviour and signalling. These functions are dependent on ECM protein composition. Since ECM defects may contribute to a broad range of disorders including cerebrovascular disease, it is crucially important to characterize ECM composition to better understand the mechanisms by which the ECM modulates brain health and disease. To date, molecular studies of the cerebrovascular ECM have been limited. We therefore generated ECM extracts from vascular enriched tissues of both mouse and human post-mortem brain. We used mass spectrometry with off-line high-pH reversed-phase fractionation to increase proteome depth and characterized the identified proteins. This identified a large number of proteins (>1000 mouse, >2000 human) in the ECM-enriched fractions, with > 66% of the identified proteins covered in both human and mouse samples. We now report 147 ECM proteins, including collagens (as major ECM components), laminins, fibronectin and nidogens, that can be considered as the core constituents of the human brain vascular matrisome. We also identified 12 novel proteins in the ECM-enriched fraction, that may have regulate or interact with the matrisome, including several key regulators of neurovascular interactions, such as BCAM, CDH5 and PARVB. Many of the identified brain vascular matrisome proteins are encoded by genes identified in stroke and cerebral small vessel disease genome-wide association studies, underscoring the importance of the vascular ECM in health and disease. This brain vascular matrisome represents a powerful resource for investigating the impact of aging and disease on the cerebrovasculature.
HostingRepository	PRIDE
AnnounceDate	2021-03-11
AnnouncementXML	Submission_2021-03-10_22:07:08.656.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raphael A Heilig
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-19 22:29:07	ID requested
⏵ 1	2021-03-10 22:07:09	announced

Dataset with its publication pending

submitter keyword: cerebrovascular, proteome, extracellular matrix, basement membrane, matrisome , ECM,

Roman Fischer
contact affiliation	Discovery Proteomics Facility University of Oxford Target Discovery Institute, NDMRB Oxford, OX3 7FZ
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Raphael A Heilig
contact affiliation	University of Oxford
contact email	raphael.heilig@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020436
     1. Label: PRIDE project
     2. Name: Global proteomic analysis of extracellular matrix in mouse and human brain highlights association with cerebrovascular disease