PXD020426

PXD020426 is an original dataset announced via ProteomeXchange.

Title	Heterotypic signaling between dermal fibroblasts and melanoma cells induces phenotypic plasticity and proteome rearrangement in malignant cells
Description	The signaling events triggered by soluble mediators released from both transformed and stromal cells shape the phenotype of tumoral cells and have significant implications in cancer development and progression. In this study we performed an in vitro heterotypic signaling assay by evaluating the proteome diversity of human dermal fibroblasts after stimulation with the conditioned media obtained from malignant melanoma cells. In addition, we also evaluated the changes in the proteome of melanoma cells after stimulation with their own conditioned media as well as with the conditioned medium from melanoma-stimulated fibroblasts. Our results pointed out to a significant rearrangement in the proteome of stromal and malignant cells upon crosstalk of soluble mediators. The main proteome signature of stimulated cells was related to protein synthesis, which may indicate that this process might be an early response of stimulated stromal cells. In addition, the conditioned medium derived from ‘primed’ stromal cells (melanoma-stimulated fibroblasts) was more effective in altering the functional phenotype (cell migration) of malignant cells than the fibroblast conditioned medium alone. Collectively, self- and cross-stimulation may play a key role in shaping the tumor microenvironment and, more importantly, enable tumoral cells to succeed in the process of melanoma progression and metastasis. Although the proteome landscape of cells participating in such a heterotypic signaling represents a snapshot of a highly dynamic state, understanding the diversity of proteins and enriched biological pathways resulting from stimulated cell states may allow for targeting specific cell regulatory motifs involved in melanoma progression and metastasis.
HostingRepository	PRIDE
AnnounceDate	2020-08-31
AnnouncementXML	Submission_2020-08-30_22:36:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andre Zelanis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-18 16:25:54	ID requested
⏵ 1	2020-08-30 22:36:21	announced

Dataset with its publication pending

submitter keyword: heterotypic signaling, melanoma, fibroblast, cancer-associated fibroblasts, phenotypic plasticity.

André Zelanis
contact affiliation	Functional Proteomics Laboratory Department of Science and Technology Federal University of São Paulo (ICT-UNIFESP) São José dos Campos, São Paulo, Brazil Rua Talim, 330, 12231-280; Tel +55 11 3385-4135 ext. 9727.
contact email	andre.zelanis@unifesp.br
lab head
Andre Zelanis
contact affiliation	Laboratory of Functional Proteomics - Department of Science and Technology - Federal University of São Paulo (UNIFESP), Sao Jose dos Campos-SP, Brazil
contact email	zelanis@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020426
     1. Label: PRIDE project
     2. Name: Heterotypic signaling between dermal fibroblasts and melanoma cells induces phenotypic plasticity and proteome rearrangement in malignant cells