PXD020418

PXD020418 is an original dataset announced via ProteomeXchange.

Title	Exploring GPCR-arrestin interfaces with genetically encoded crosslinkers
Description	-arrestins (arr1 and arr2) are ubiquitous regulators of G protein-coupled receptor (GPCR) signalling. Available data suggest that -arrestins dock to different receptors in different ways. However, the structural characterization of arrestin-GPCR complexes is challenging and alternative approaches to study arrestin-GPCR complexes are needed. Here, starting from the finger loop as a major site for the interaction of arrestins with GPCRs, we genetically incorporate non-canonical amino acids for photo- and chemical crosslinking into arr1 and arr2 and explore binding topologies to GPCRs forming either stable or transient complexes with arrestin: the vasopressin receptor 2 (rhodopsin-like), the corticotropin releasing factor receptor 1 and the parathyroid hormone receptor 1 (both secretin-like). We show that each receptor leaves a unique footprint on arrestin, whereas the two -arrestins yield quite similar crosslinking patterns. Furthermore, we show that the method allows defining the orientation of arrestin respect to the GPCR. Finally, we provide direct evidence for the formation of arrestin oligomers in the cell.
HostingRepository	PRIDE
AnnounceDate	2020-09-21
AnnouncementXML	Submission_2020-09-20_23:07:29.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020418
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Ihling
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-17 09:06:33	ID requested
⏵ 1	2020-09-20 23:07:30	announced

B, ö, ttke T, Ernicke S, Serfling R, Ihling C, Burda E, Gurevich VV, Sinz A, Coin I, Exploring GPCR-arrestin interfaces with genetically encoded crosslinkers. EMBO Rep, 21(11):e50437(2020) [pubmed]

submitter keyword: -arrestins / genetically encoded crosslinkers / G protein-coupled receptor / GPCR-arrestin complexes / live cells

Andrea Sinz
contact affiliation	Department of Pharmaceutical Chemistry & Bioanalytics Institute of Pharmacy Martin-Luther University Halle-Wittenberg Germany
contact email	andrea.sinz@pharmazie.uni-halle.de
lab head
Christian Ihling
contact affiliation	MLU Halle, Inst. f. Pharmacy
contact email	christian.ihling@pharmazie.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020418
     1. Label: PRIDE project
     2. Name: Exploring GPCR-arrestin interfaces with genetically encoded crosslinkers