PXD020414

PXD020414 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomic analysis of insect larval and metamorphic molts
Description	Molting is an essential biological process occurring characteristic times throughout the life cycle of holometabolous insects. However, it is not clear how insects determine whether the direction of molting is to remain status quo or to initiate metamorphosis. Here, we used liquid chromatography-mass spectrometry to identify the molecules involved in larval and metamorphic molting, and compare the differentially expressed proteins (DEPs) in the two processes, to explore the functional factors that determine the direction of molts. There were 322 and 1140 DEPs identified in larval and metamorphic molting process, respectively. Bioinformatics analyses show that the amino sugar pathway was up-regulated in both processes. The up-regulated protease contributed to the metamorphosis. In addition, several proteins with different expression patterns in larval-larval and larval-pupal transitions, including Endochitinase, GRIM-19 (Genes associated with retinoid-IFN-induced mortality-19), IDE (Insulin-degrading enzyme), Sorcin (Soluble resistance related calcium binding protein), OBP (Odorant-binding protein-2 precursor), TRAP1(Tumor necrosis factor receptor associated protein-1), etc., were further identified by parallel reaction monitoring, which may play diverse functions in the determination of molting properties. These results provide a proteomic insight into molecules involved in larval and metamorphic molts, and will likely improve the current understanding of determination of direction of molts.
HostingRepository	PRIDE
AnnounceDate	2020-10-30
AnnouncementXML	Submission_2020-10-30_01:16:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dujuan Dong
SpeciesList	scientific name: Helicoverpa armigera armigera; NCBI TaxID: 52318;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-17 04:10:09	ID requested
⏵ 1	2020-10-30 01:16:41	announced

Dataset with its publication pending

submitter keyword: holometabolous insects

cotton worm

LC-MS/MS

Dujuan Dong
contact affiliation	Shandong University
contact email	dongdujuan@163.com
lab head
Dujuan Dong
contact affiliation	Shandong University
contact email	dongdujuan@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020414
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomic analysis of insect larval and metamorphic molts