PXD020408

PXD020408 is an original dataset announced via ProteomeXchange.

Title	Proteomics studies confirm CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana
Description	Insecticide resistance is a worldwide threat for vector control around the world, and Aedes aegypti , the main vector of several arboviruses, is a particular concern. To better understand the mechanisms of resistance, four isofemale strains originally from French Guiana were isolated and analysed using combined approaches. The activity of detoxification enzymes involved in insecticide resistance was assayed, and mutations located at positions 1016 and 1534 of the sodium voltage-gated channel gene, which have been associated with pyrethroid resistance in Aedes aegypti populations in Latin America, were monitored. Resistance to other insecticide families (organophosphates and carbamates) was evaluated. A large-scale proteomic analysis was performed to identify proteins involved in insecticide resistance. Our results revealed a metabolic resistance and resistance associated with a mutation of the sodium voltage-gated channel gene at position 1016. Metabolic resistance was mediated through an increase of esterase activity in most strains but also through the shifts in the abundance of several cytochrome P450 (CYP450s). Overall, resistance to deltamethrin was linked in the isofemale strains to resistance to other class of insecticides, suggesting that cross- and multiple resistance occur through selection of mechanisms of metabolic resistance. These results give some insights into resistance to deltamethrin and into multiple resistance phenomena in populations of Ae. aegypti
HostingRepository	PRIDE
AnnounceDate	2020-12-09
AnnouncementXML	Submission_2021-01-17_23:18:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIETTE MATONDO
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-16 22:50:25	ID requested
1	2020-12-09 06:51:50	announced
⏵ 2	2021-01-17 23:18:44	announced	2021-01-18: Updated publication reference for PubMed record(s): 33428654.

Epelboin Y, Wang L, Giai Gianetto Q, Choumet V, Gaborit P, Issaly J, Guidez A, Douch, é T, Chaze T, Matondo M, Dusfour I, CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies. PLoS One, 16(1):e0243992(2021) [pubmed]

submitter keyword: Aedes aegypti, insecticide resistance, multiple resistance, CYP, diagnostic concentration

Isabelle Dusfour
contact affiliation	Institut Pasteur de la Guyane, Vectopôle Amazonien "Emile Abonnenc", Vector Adaptation and Control Unit, Cayenne, French Guiana, France INRS-Centre Armand Frappier Santé Biotechnologie, Groupe de recherche en écologie microbienne, H7V 1B7, QC, Canada Global Health department, Institut Pasteur, Paris
contact email	isabelle.dusfour@pasteur.fr
lab head
MARIETTE MATONDO
contact affiliation	Pasteur
contact email	mariette.matondo@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD020408

PRIDE project URI

• PRIDE
  1. PXD020408
     1. Label: PRIDE project
     2. Name: Proteomics studies confirm CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana