PXD020402

PXD020402 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison of photosynthetic reaction centre-light harvesting 1 complexes in wild-type, protein-W knockout and His-tagged protein-W strains of Rhodopseudomonas palustris.
Description	Rhodopseudomonas palustris is a species of purple phototrophic bacterium. In these species, solar energy is captured by reaction centre-light harvesting 1 (RC-LH1) complexes which reside in membranes within the cells. The RC comprises three protein subunits: H, M, L and is encircled by a ring of LH1-α and LH1-β subunit pairs. Approximately 10% of these complexes in the wild-type (WT) strain include an additional subunit called protein-W. In this project, we have determined cryo-EM structures for RC-LH1 complexes both with and without protein-W. To enable the purification of RC-LH1-W with 100% occupancy of protein-W, strain expressing a C-terminally His-tagged W was engineered. For complexes lacking W, a knockout strain was used. Proteomic analysis was employed to validate these strains and establish that the WT and W-His expressed similar levels of W relative to RC-LH1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:37.312.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Rhodopseudomonas palustris CGA009; NCBI TaxID: 258594;
ModificationList	methylthiolated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-16 07:27:23	ID requested
1	2021-09-09 02:59:35	announced
⏵ 2	2024-10-22 05:28:45	announced	2024-10-22: Updated project metadata.

10.1126/sciadv.abe2631;

Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN, RC-LH1 complexes with open or closed quinone channels. Sci Adv, 7(3):(2021) [pubmed]

submitter keyword: LH1-β, LH1-α, photosynthetic reaction centre-light harvesting 1 complex, protein-W,Rhodopseudomonas palustris

Christopher Neil Hunter
contact affiliation	Department of Molecular Biology and Biotechnology, The University of Sheffield, UK.
contact email	c.n.hunter@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020402

PRIDE project URI

• PRIDE
  1. PXD020402
     1. Label: PRIDE project
     2. Name: Proteomic comparison of photosynthetic reaction centre-light harvesting 1 complexes in wild-type, protein-W knockout and His-tagged protein-W strains of Rhodopseudomonas palustris.