PXD020397

PXD020397 is an original dataset announced via ProteomeXchange.

Title	Periodontal pathogens do not exacerbate macroscopic arthritis but alter the synovial proteome
Description	Rheumatoid arthritis (RA) and periodontitis (PD) are chronic inflammatory diseases that appear to occur in tandem. Autoantibodies against citrullinated peptide antigens linked pathogeneses with PD preceding RA. However, the mutual impact PD exerts on RA and vice versa has not yet been defined. To address this issue, we set up an animal model and analyzed how the prime inducers of citrullination - Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans – differ in their pathogenic potential. Our experimental setup included collagen induced arthritis (CIA) in the mouse, oral inoculation with P. gingivalis or A. actinomycetemcomitans to induce alveolar bone loss and the combination of both diseases in inverted orders of events. Label-free quantitative proteome analysis revealed that P. gingivalis and A. actinomycetemcomitans led to differential expression patterns in the synovial membranes that were reminiscent of cellular and humoral immune responses, respectively. The P. gingivalis and A. actinomycetemcomitans specific signatures in the synovial proteomes suggest a role for oral pathogens in shaping disease subtypes and setting the stage for subsequent therapy response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:12.690.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020397
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stefan Mikkat
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-16 04:19:51	ID requested
1	2020-12-04 06:09:09	announced
⏵ 2	2024-10-22 05:16:13	announced	2024-10-22: Updated project metadata.

10.6019/PXD020397;

submitter keyword: Collagen-induced arthritis, HDMSE, Mus musculus,Rheumatoid arthritis, Synovial membrane, Periodontitis

Brigitte Mueller-Hilke
contact affiliation	Core Facility for Cell Sorting & Cell Analysis, Laboratory for Clinical Immunology, University Medical Center Rostock, 18057 Rostock, Schillingallee 70, Germany
contact email	brigitte.mueller-hilke@med.uni-rostock.de
lab head
Stefan Mikkat
contact affiliation	Core Facility Proteome Analysis, University Medicine Rostock, Schillingallee 69, 18057 Rostock
contact email	stefan.mikkat@med.uni-rostock.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD020397

PRIDE project URI

• PRIDE
  1. PXD020397
     1. Label: PRIDE project
     2. Name: Periodontal pathogens do not exacerbate macroscopic arthritis but alter the synovial proteome