PXD020387

PXD020387 is an original dataset announced via ProteomeXchange.

Title	N-glycosylation analysis of human alpha-1-acid glycoprotein LC-MS
Description	Alpha-1-acid glycoprotein (AGP) is an acute phase glycoprotein in blood, which is primarily synthetized in the liver and whose biological role is not completely understood. It consists 45% of carbohydrates attached in the form of five N-linked complex glycans. AGP N-glycosylation was shown to be changed in many different diseases and some changes appear to be disease-specific, thus it has a great diagnostic and prognostic potential. However, AGP glycosylation was mainly analyzed in small cohorts and without detailed site-specific glycan information. Here, we developed a cost-effective method for a high-throughput and site-specific N-glycosylation LC-MS analysis of AGP which can be applied on large cohorts, aid in search for novel disease biomarkers and enable better understanding of AGP’s role and function in health and disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:21.331.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Tijardović
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	compact

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-15 15:36:33	ID requested
1	2021-01-04 06:18:03	announced
⏵ 2	2024-10-22 05:17:22	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra120.002433;

submitter keyword: AGP, LC-MS, high troughput,N-glycosylation

Olga Gornik Kljaić
contact affiliation	Faculty of Pharmacy and Biochemistry, University of Zagreb
contact email	ogornik@pharma.unizg.hr
lab head
Marko Tijardović
contact affiliation	Faculty of Pharmacy and Biochemistry, University of Zagreb
contact email	mtijardovic@pharma.hr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD020387

PRIDE project URI

• PRIDE
  1. PXD020387
     1. Label: PRIDE project
     2. Name: N-glycosylation analysis of human alpha-1-acid glycoprotein LC-MS