PXD020384

PXD020384 is an original dataset announced via ProteomeXchange.

Title	Hydrogen sulfide sensitizes Acinetobacter baumannii to killing by antibiotics.
Description	The production of endogenous hydrogen sulfide (H2S) has been shown to confer antibiotic tolerance in all bacteria studied to date. Therefore, this mediator has been speculated to be a universal defense mechanism against antibiotics in bacteria. This is assuming that all bacteria produce endogenous H2S. In this study, we established that the pathogenic bacteria Acinetobacter baumannii does not produce endogenous H2S, giving us the opportunity to test the effect of exogenous H2S on antibiotic tolerance in a bacterium that does not produce it. By using a H2S-releasing compound to modulate the sulfide content in A. baumannii, we demonstrated that instead of conferring antibiotic tolerance, exogenous H2S sensitized A. baumannii to multiple antibiotic classes, and was able to revert acquired resistance to gentamicin. Exogenous H2S triggered a perturbation of redox and energy homeostasis that translated into hypersensitivity to antibiotic killing. We propose that H2S could be used as an antibiotic-potentiator and resistance-reversion agent in bacteria that do not produce it.
HostingRepository	PRIDE
AnnounceDate	2021-06-08
AnnouncementXML	Submission_2021-06-07_21:54:44.452.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020384
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	AMEYA SINHA
SpeciesList	scientific name: Acinetobacter baumannii AB_TG2631; NCBI TaxID: 1221282;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-15 07:49:39	ID requested
1	2021-06-07 18:18:33	announced
⏵ 2	2021-06-07 21:54:45	announced	2021-06-08: Updated publication reference for PubMed record(s): 32849459.

Ng SY, Ong KX, Surendran ST, Sinha A, Lai JJH, Chen J, Liang J, Tay LKS, Cui L, Loo HL, Ho P, Han J, Moreira W, to Killing by Antibiotics. Front Microbiol, 11():1875(2020) [pubmed]

submitter keyword: Acinetobacter baumannii, Hydrogen sulfide, AMR

Wilfried Moreira
contact affiliation	Director
contact email	moreirawilfried@gmail.com
lab head
AMEYA SINHA
contact affiliation	NTU
contact email	ameya.sinha@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020384
     1. Label: PRIDE project
     2. Name: Hydrogen sulfide sensitizes Acinetobacter baumannii to killing by antibiotics.