PXD020379 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tissue- and isoform-specific protein complex analysis with natively processed bait proteins |
Description | Protein-protein interaction analysis is an important tool to elucidate the function of proteins and protein complexes as well as their dynamic behavior. To date, the analysis of tissue- or even cell- or compartment-specific protein interactions is still relying on the availability of specific antibodies suited for immunoprecipitation. Here, we aimed at establishing a method that allows identification of protein interactions and complexes from intact tissues independent of specific, high affinity antibodies used for protein pull-down and isolation. Tagged bait proteins were expressed in human HEK293T cells and residual interactors removed by SDS. The resulting tag-fusion protein was then used as bait to pull proteins from tissue samples. Tissue-specific interactions were reproducibly identified from porcine retina as well as from retinal pigment epithelium using the ciliary protein lebercilin as bait. Further, murine heart-specific interactors of two gene products of the 3’,5’-cyclic guanosine-3’,5’-monophosphate (cGMP)-dependent protein kinase type I were investigated. Here, specific interactions were associated with the Iα and Iβ gene products, that differ only in their unique amino-terminal region comprising about 100 AS. As such, the new protocol provides a fast and reliable method for tissue-specific protein complex analysis which is independent of the availability or suitability of antibodies for immunoprecipitation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:18:55.819.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tina Beyer |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-15 07:09:06 | ID requested | |
1 | 2023-12-28 11:36:42 | announced | |
⏵ 2 | 2024-10-22 06:18:56 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Flag tag,tissue-specific complex |
Contact List
Marius Ueffing |
contact affiliation | Institute for Ophthalmic Research Centre for Ophthalmology, University of Tübingen, Germany |
contact email | marius.ueffing@uni-tuebingen.de |
lab head | |
Tina Beyer |
contact affiliation | Uniklinik Tübingen |
contact email | tina.beyer@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020379
- Label: PRIDE project
- Name: Tissue- and isoform-specific protein complex analysis with natively processed bait proteins