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PXD020379

PXD020379 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTissue- and isoform-specific protein complex analysis with natively processed bait proteins
DescriptionProtein-protein interaction analysis is an important tool to elucidate the function of proteins and protein complexes as well as their dynamic behavior. To date, the analysis of tissue- or even cell- or compartment-specific protein interactions is still relying on the availability of specific antibodies suited for immunoprecipitation. Here, we aimed at establishing a method that allows identification of protein interactions and complexes from intact tissues independent of specific, high affinity antibodies used for protein pull-down and isolation. Tagged bait proteins were expressed in human HEK293T cells and residual interactors removed by SDS. The resulting tag-fusion protein was then used as bait to pull proteins from tissue samples. Tissue-specific interactions were reproducibly identified from porcine retina as well as from retinal pigment epithelium using the ciliary protein lebercilin as bait. Further, murine heart-specific interactors of two gene products of the 3’,5’-cyclic guanosine-3’,5’-monophosphate (cGMP)-dependent protein kinase type I were investigated. Here, specific interactions were associated with the Iα and Iβ gene products, that differ only in their unique amino-terminal region comprising about 100 AS. As such, the new protocol provides a fast and reliable method for tissue-specific protein complex analysis which is independent of the availability or suitability of antibodies for immunoprecipitation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:18:55.819.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTina Beyer
SpeciesList scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-15 07:09:06ID requested
12023-12-28 11:36:42announced
22024-10-22 06:18:56announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.jprot.2020.103947;
Keyword List
submitter keyword: Flag tag,tissue-specific complex
Contact List
Marius Ueffing
contact affiliationInstitute for Ophthalmic Research Centre for Ophthalmology, University of Tübingen, Germany
contact emailmarius.ueffing@uni-tuebingen.de
lab head
Tina Beyer
contact affiliationUniklinik Tübingen
contact emailtina.beyer@uni-tuebingen.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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