PXD020379

PXD020379 is an original dataset announced via ProteomeXchange.

Title	Tissue- and isoform-specific protein complex analysis with natively processed bait proteins
Description	Protein-protein interaction analysis is an important tool to elucidate the function of proteins and protein complexes as well as their dynamic behavior. To date, the analysis of tissue- or even cell- or compartment-specific protein interactions is still relying on the availability of specific antibodies suited for immunoprecipitation. Here, we aimed at establishing a method that allows identification of protein interactions and complexes from intact tissues independent of specific, high affinity antibodies used for protein pull-down and isolation. Tagged bait proteins were expressed in human HEK293T cells and residual interactors removed by SDS. The resulting tag-fusion protein was then used as bait to pull proteins from tissue samples. Tissue-specific interactions were reproducibly identified from porcine retina as well as from retinal pigment epithelium using the ciliary protein lebercilin as bait. Further, murine heart-specific interactors of two gene products of the 3’,5’-cyclic guanosine-3’,5’-monophosphate (cGMP)-dependent protein kinase type I were investigated. Here, specific interactions were associated with the Iα and Iβ gene products, that differ only in their unique amino-terminal region comprising about 100 AS. As such, the new protocol provides a fast and reliable method for tissue-specific protein complex analysis which is independent of the availability or suitability of antibodies for immunoprecipitation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:55.819.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tina Beyer
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-15 07:09:06	ID requested
1	2023-12-28 11:36:42	announced
⏵ 2	2024-10-22 06:18:56	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2020.103947;

Beyer T, Klose F, Kuret A, Hoffmann F, Lukowski R, Ueffing M, Boldt K, Tissue- and isoform-specific protein complex analysis with natively processed bait proteins. J Proteomics, 231():103947(2021) [pubmed]

submitter keyword: Flag tag,tissue-specific complex

Marius Ueffing
contact affiliation	Institute for Ophthalmic Research Centre for Ophthalmology, University of Tübingen, Germany
contact email	marius.ueffing@uni-tuebingen.de
lab head
Tina Beyer
contact affiliation	Uniklinik Tübingen
contact email	tina.beyer@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD020379

PRIDE project URI

• PRIDE
  1. PXD020379
     1. Label: PRIDE project
     2. Name: Tissue- and isoform-specific protein complex analysis with natively processed bait proteins