PXD020377

PXD020377 is an original dataset announced via ProteomeXchange.

Title	The proteomic inventory reveals the chloroplast ribosome as nexus within a diverse protein network
Description	In plant cells, chloroplast gene expression is predominantly controlled through regulation of translation. Fine-tuning of protein synthesis is vital for plant development, acclimation to environmental challenges and for the assembly of major protein complexes such as the photosynthesis machinery. However, little is known about the degree and nature of the regulatory network, mainly due to challenges associated with the specific isolation of chloroplast ribosomes under conditions that also retain weak or transient interactions. Here, we established a ribosome affinity purification method, which enabled us to map the global interaction network of chloroplast ribosomes. Endogenously tagging a protein of the large or small subunit revealed not only interactors of the holo complex, but also preferential interactors of the two subunits. This includes known canonical regulatory proteins as well as several new proteins belonging to the categories of protein and RNA regulation, photosystem biogenesis, redox control and plastidic metabolism. By further characterizing identified proteins, we provide the first evidence for the existence of a co-translational N-acetyltransferase process in chloroplasts. Our dataset establishes the chloroplast ribosome as nexus in a highly choreographed, spatially interconnected protein network and reveals its wide-ranging regulatory potential during gene expression.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-04-06_23:26:13.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robin Roth
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-15 06:52:48	ID requested
1	2021-01-03 22:59:35	announced
⏵ 2	2021-04-06 23:26:14	announced	2021-04-07: Updated publication reference for PubMed record(s): 33330923.

Westrich LD, Gotsmann VL, Herkt C, Ries F, Kazek T, Tr, ö, sch R, Armbruster L, M, ü, hlenbeck JS, Ramundo S, Nickelsen J, Finkemeier I, Wirtz M, Storchov, á Z, R, ä, schle M, Willmund F, The versatile interactome of chloroplast ribosomes revealed by affinity purification mass spectrometry. Nucleic Acids Res, 49(1):400-415(2021) [pubmed]

submitter keyword: Chloroplast, Ribosome, LCMS, Chlamydomonas Rheinhardtii

Zuzana Storchova
contact affiliation	Technische Universität Kaiserslautern
contact email	storchova@bio.uni-kl.de
lab head
Robin Roth
contact affiliation	Technische Universität Kaiserslautern
contact email	rroth@bio.uni-kl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020377
     1. Label: PRIDE project
     2. Name: The proteomic inventory reveals the chloroplast ribosome as nexus within a diverse protein network