PXD020374

PXD020374 is an original dataset announced via ProteomeXchange.

Title	Collagen-derived prolyl-hydroxyproline dipeptide promotes cellular homeostasis and lamellipodia-driven cell motility through active β1-integrin in adult tendon cells
Description	Collagen-derived hydroxyproline-containing peptides show a variety of biological properties in cells. However, no comprehensive study has yet explored the functional links between Hyp-containing peptides and cellular behavior. Here, we show that the dipeptide prolyl-hydroxyproline (Pro–Hyp) exhibits the most significant effect of a number of di- and tri-peptides on mouse tendon cells. We have shown that the cellular uptake of Pro-Hyp is a carrier-mediated process. In addition, Pro-Hyp promotes differentiation/maturation of tendon cells with modulation of lineage-specific factors, has profound effects on cellular phenotypes such as cell proliferation with significantly upregulated ERK-phosphorylation and extracellular matrix production with increased type I collagen network organization, and induced significant chemotactic activity in vitro. Proteomics analysis has identified cellular assembly and organizations, and movement as predicted linked-network pathways in response to Pro-Hyp. Mechanistically, cells treated with Pro-Hyp demonstrate increased directional persistence and significantly increased directed motility and migration velocity, which are accompanied by elongated lamellipodial protrusions with increased active β1-integrin-containing focal contacts and reorganizations of thicker peripheral F-actin fibrils. Thus, our findings document the molecular basis of the functional benefits of Pro-Hyp dipeptide in cellular behavior. Such dynamic properties of collagen-derived Pro-Hyp dipeptide could lead the way to its application to translational medicine.
HostingRepository	PRIDE
AnnounceDate	2022-09-28
AnnouncementXML	Submission_2022-09-28_06:40:59.304.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rosalind Jenkins
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-15 06:42:00	ID requested
⏵ 1	2022-09-28 06:41:00	announced

Ide K, Takahashi S, Sakai K, Taga Y, Ueno T, Dickens D, Jenkins R, Falciani F, Sasaki T, Ooi K, Kawashiri S, Mizuno K, Hattori S, Sakai T, 1-integrin in adult tendon cells. J Biol Chem, 297(1):100819(2021) [pubmed]

submitter keyword: Collagen-derived peptide

Proline-hydroxyproline

Adult tendon cells

Extracellular matrix

Scleraxis

Cell motility

Extracellular signal-regulated kinase

Rosalind E Jenkins
contact affiliation	Centre for Drug Safety Science, Dept Pharmacology and Therapeutics, Institute for Systems, Molecular and Integrative Biology, University of Liverpool
contact email	R.Jenkins@liv.ac.uk
lab head
Rosalind Jenkins
contact affiliation	University of Liverpool
contact email	R.Jenkins@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020374
     1. Label: PRIDE project
     2. Name: Collagen-derived prolyl-hydroxyproline dipeptide promotes cellular homeostasis and lamellipodia-driven cell motility through active β1-integrin in adult tendon cells