PXD020362

PXD020362 is an original dataset announced via ProteomeXchange.

Title	MS imaging-guided microproteomics for spatial omics on a single instrument
Description	Mass spectrometry imaging (MSI) allows investigating the spatial distribution of chemical compounds directly in biological tissues. As the analytical depth of MSI is limited, MSI needs to be coupled to more sensitive local extraction-based omics approaches to achieve a comprehensive molecular characterization. For this it is important to retain the spatial information provided by MSI for follow-up omics studies. It has been shown that regiospecific MSI data can be used to guide a laser microdissection system (LMD) for ultra-sensitive LC-MS analyses. So far, this combination has required separate and specialized MS instrumentation. Recent advances in dual-source instrumentation, harboring both MALDI and ESI sources, promise state-of-the-art MSI and liquid-based proteomic capabilities on the same MS instrument. In this study, we demonstrate that such an instrument can offer both, fast lipid-based MSI at high mass- and high lateral resolution, and sensitive LC-MS on local protein extracts from the exact same tissue section.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:15:10.027.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Balluff
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-14 06:05:38	ID requested
1	2020-11-16 14:16:47	announced
⏵ 2	2024-10-22 05:15:11	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201900369;

Dewez F, Oejten J, Henkel C, Hebeler R, Neuweger H, De Pauw E, Heeren RMA, Balluff B, MS Imaging-Guided Microproteomics for Spatial Omics on a Single Instrument. Proteomics, 20(23):e1900369(2020) [pubmed]

submitter keyword: spatial omics, microproteomics,laser microdissection, mass spectrometry imaging

Benjamin Balluff
contact affiliation	M4I Division of Imaging Mass Spectrometry, Maastricht, The Netherlands
contact email	b.balluff@maastrichtuniversity.nl
lab head
Benjamin Balluff
contact affiliation	Maastricht University
contact email	b.balluff@maastrichtuniversity.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD020362

PRIDE project URI

• PRIDE
  1. PXD020362
     1. Label: PRIDE project
     2. Name: MS imaging-guided microproteomics for spatial omics on a single instrument