PXD020361

PXD020361 is an original dataset announced via ProteomeXchange.

Title	Aerobic and anaerobic hydrogen respiratory pathways in the extreme acidophile Acidithiobacillus ferrooxidans
Description	Hydrogen can be an important source of energy for chemolithotrophic acidophiles, especially in the deep terrestrial subsurface. Nevertheless, the current knowledge of microbial hydrogen utilization in acidic environments is minimal. A multi-omics analysis was applied on Acidithiobacillus ferrooxidans growing aerobically and anaerobically (with ferric iron) on hydrogen as an electron donor, and a respiratory model proposed from the results obtained. In this model, both [NiFe] hydrogenases, cytoplasmic uptake and membrane-bound respiratory, oxidize molecular hydrogen to two protons and two electrons. The electrons are used to reduce membrane-soluble ubiquinone to ubiquinol. Genetically associated [FeS]-binding proteins mediate electron relay from the hydrogenases to the ubiquinone pool. Under aerobic conditions, reduced ubiquinol transfers electrons to either cytochrome aa3 oxidase via cytochrome bc1 complex and cytochrome c4 or the alternate directly to cytochrome bd oxidase, resulting in proton efflux together with the reduction of molecular oxygen to water. Under anaerobic conditions, reduced ubiquinol transfers electrons to outer membrane cytochrome c (ferric iron reductase) via cytochrome bc1 complex and a cascade of electron transporters (cytochrome c4, cytochrome c552, rusticyanin, and high potential iron-sulfur protein), resulting in proton efflux together with the reduction of ferric iron to ferrous iron. The proton gradient generated by molecular hydrogen oxidation maintains the membrane potential and allows the generation of ATP via ATP synthase and NADH via NADH-ubiquinone oxidoreductase. To a lesser extent, NADH can also be generated by another bidirectional cytoplasmic hydrogenase. ATP and NADH are further utilized in the Calvin–Benson–Bassham cycle for inorganic carbon uptake and assimilation. These results further clarify the role of extremophiles in biogeochemical processes and their impact on the composition and features of the deep terrestrial subsurface from the distant past to the present.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:15:37.853.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Bouchal
SpeciesList	scientific name: Acidithiobacillus ferrooxidans; NCBI TaxID: 920;
ModificationList	iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-14 05:59:32	ID requested
1	2020-11-23 23:27:46	announced
⏵ 2	2024-10-22 05:15:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Acidithiobacillus ferrooxidans

acidophilic bacteria

iron reduction

hydrogen oxidation

hydrogenase

oxygen reduction

quantitative proteomics

respiratory pathways

RNA sequencing

Rubisco

Jiri Kucera
contact affiliation	Masaryk University, Faculty of Science, Department of Biochemistry
contact email	jiri.kucera@sci.muni.cz
lab head
Pavel Bouchal
contact affiliation	Masaryk University, Faculty of Science
contact email	bouchal@chemi.muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020361
     1. Label: PRIDE project
     2. Name: Aerobic and anaerobic hydrogen respiratory pathways in the extreme acidophile Acidithiobacillus ferrooxidans