PXD020359 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isolation and mass spectrometry analysis of Atg9 vesicles from Saccharomyces cerevisiae |
Description | Autophagy is a eukaryotic bulk degradation pathway that allows cells to degrade potentially harmful cytosolic components or to provide necessary nutrients during starvation. This cargo degradation is achieved by its sequestration within double membrane vesicles termed autophagosomes, which fuse with the vacuole where it is degraded. Atg (autophagy-related) proteins are the main group of proteins involved in this process and, interestingly, Atg9 is the only integral membrane yeast Atg protein absolutely required for autophagy progression. In the cell it resides in Golgi-derived vesicles, which are indispensable for the nucleation of the autophagosome. There is not much known about biochemical properties of these vesicles in particular their protein content apart from Atg9. To address this question and to identify putative interaction partners of Atg9, the Atg9-vesicles were isolated and submitted to mass spectrometry analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-03 |
AnnouncementXML | Submission_2022-03-03_00:45:51.122.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Markus Hartl |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-14 05:02:08 | ID requested | |
⏵ 1 | 2022-03-03 00:45:52 | announced | |
Publication List
Sawa-Makarska J, Baumann V, Coudevylle N, von B, ΓΌ, low S, Nogellova V, Abert C, Schuschnig M, Graef M, Hummer G, Martens S, Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation. Science, 369(6508):(2020) [pubmed] |
Keyword List
submitter keyword: yeast, autophagy, Atg9, vesicles |
Contact List
Sascha Martens |
contact affiliation | Max Perutz Labs Vienna, Department of Biochemistry and Cell Biology, University of Vienna, Vienna BioCenter, Austria |
contact email | sascha.martens@univie.ac.at |
lab head | |
Markus Hartl |
contact affiliation | Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 3, 1030 Vienna |
contact email | markus.hartl@univie.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020359
- Label: PRIDE project
- Name: Isolation and mass spectrometry analysis of Atg9 vesicles from Saccharomyces cerevisiae