PXD020353

PXD020353 is an original dataset announced via ProteomeXchange.

Title	Accelerated Lysis and Proteolytic Digestion of Biopsy-level Fresh Frozen and FFPE Tissue Samples Using Pressure Cycling Technology
Description	We developed an accelerated PCT method with doubled throughput while maintaining digestion efficiency, the number of proteins/peptides identified, as well as reproducibility of protein quantitation, compared to the previously reported method. The accelerated PCT method does not require new chemical reagents or increase the cost of reagents. This accelerated PCT method simplifies the manual sample handling steps, and thereby reduces user error and technical variability. The method has been benchmarked and validated using both FFPE and FF mouse liver tissue samples.
HostingRepository	iProX
AnnounceDate	2020-07-16
AnnouncementXML	Submission_2020-07-16_19:04:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tiannan Guo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-14 01:47:36	ID requested
⏵ 1	2020-07-16 19:04:07	announced

Gao H, Zhang F, Liang S, Zhang Q, Lyu M, Qian L, Liu W, Ge W, Chen C, Yi X, Zhu J, Lu C, Sun P, Liu K, Zhu Y, Guo T, Accelerated Lysis and Proteolytic Digestion of Biopsy-Level Fresh-Frozen and FFPE Tissue Samples Using Pressure Cycling Technology. J Proteome Res, 19(5):1982-1990(2020) [pubmed]

submitter keyword: PCT2G, hepatocellular carcinoma tissue, PulseDIA, 10plexTMT

Tiannan Guo
contact affiliation	Westlake Unveristy
contact email	Tiannan.guo@hotmail.com
lab head
Tiannan Guo
contact affiliation	Westlake University
contact email	Tiannan.guo@hotmail.com
dataset submitter

iProX dataset URI