PXD020344 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | N-glycoproteins have a major role in MGL binding to colorectal cancer cell lines |
Description | Colorectal cancer (CRC) is the second leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease. For this reason, many efforts have been made towards new approaches for early detection and prognosis. Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis. We had recently identified the major MGL binding glycoproteins in two high MGL binding CRC cells lines, HCT116 and HT29. We investigated here the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL. In addition, we performed quantitative proteomics to study the major differences in proteins involved in glycosylation in these cells. Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-10 |
AnnouncementXML | Submission_2020-08-09_22:59:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yassene Mohammed |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-13 17:44:12 | ID requested | |
⏵ 1 | 2020-08-09 22:59:42 | announced | |
Publication List
Pirro M, Mohammed Y, van Vliet SJ, Rombouts Y, Sciacca A, de Ru AH, Janssen GMC, Tjokrodirijo RTN, Wuhrer M, van Veelen PA, Hensbergen PJ, -Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity. Int J Mol Sci, 21(15):(2020) [pubmed] |
Keyword List
submitter keyword: Glycoproteomics |
TMT labelling |
C-type lectin |
Colorectal cancer |
LacdiNAc |
Tn antigen |
Contact List
Paul J. Hensbergen |
contact affiliation | Center for Proteomics and Metabolomics Leiden University Medical Center |
contact email | P.J.Hensbergen@lumc.nl |
lab head | |
Yassene Mohammed |
contact affiliation | LUMC/UVIC |
contact email | y.mohammed@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020344
- Label: PRIDE project
- Name: N-glycoproteins have a major role in MGL binding to colorectal cancer cell lines