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PXD020340

PXD020340 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets
DescriptionPoly(ADP-ribose) polymerase 7 (PARP7) has emerged as a critically important member of a large enzyme family that catalyze mono-ADP-ribosylation (MARylation) in mammalian cells. Unlike other PARPs, PARP7 is expressed in many different cell types and is upregulated by diverse stimuli, such as toxins, growth factors, steroid hormones, and immunomodulatory ligands. Recent evidence by several independent studies show that PARP7 is a critical regulator of the innate immune response. What remains unclear is the mechanism by which PARP7 regulates this process as well as other cellular processes. This is because the protein targets, and the specific amino acid sites, of PARP7 MARylation are largely unknown. Here, we combine chemical genetics, proximity labeling, and proteome-wide amino acid ADP-ribosylation (ADPr) site profiling for identifying the direct targets and sites of PARP7-mediated MARylation. We found that the inactive PARP family member, PARP13—a critical regulator of the innate antiviral immune response—is a major target of PARP7, and is preferentially MARylated on cysteine residues in its RNA binding zinc finger domain. Proteome-wide MARylation analysis reveals cysteine as a major ADPr acceptor of PARP7. These studies not only provide insight into PARP7 targeting and MARylation site preference, but also serve as an important resource for further understanding PARP7 function in the innate immune response and other pathways.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:30:09.634.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSara Larsen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListadenosine diphosphoribosyl (ADP-ribosyl) modified residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-13 17:22:41ID requested
12021-11-04 00:21:14announced
22024-10-22 05:30:10announced2024-10-22: Updated project metadata.
Publication List
10.7554/elife.60480;
Rodriguez KM, Buch-Larsen SC, Kirby IT, Siordia IR, Hutin D, Rasmussen M, Grant DM, David LL, Matthews J, Nielsen ML, Cohen MS, Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets. Elife, 10():(2021) [pubmed]
Keyword List
submitter keyword: ADPr, PTM, Post-translational modification, PARP7,ADP-ribosylation
Contact List
Michael Lund Nielsen
contact affiliationProteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark
contact emailmichael.lund.nielsen@cpr.ku.dk
lab head
Sara Larsen
contact affiliationNNF Center for Protein Research
contact emailsara.larsen@cpr.ku.dk
dataset submitter
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