PXD020332

PXD020332 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of Corallium rubrum biominerals
Description	The process of calcium carbonate biomineralization has arisen multiple times during metazoan evolution. In the phylum Cnidaria, biomineralization has mostly been studied in the subclass Hexacorallia (i.e. stony corals) in comparison to the subclass Octocorallia (i.e. red corals); the two diverged approximately 600 million years ago. The precious Mediterranean red coral, Corallium rubrum, is an octocorallian species, which produces two distinct high-magnesium calcite biominerals, the axial skeleton and the sclerites. In order to gain insight into the red coral biomineralization process and cnidarian biomineralization evolution, we studied the protein repertoire forming the organic matrix (OM) of its two biominerals. We combined High-Resolution Mass Spectrometry and transcriptome analysis to study the OM composition of the axial skeleton and the sclerites. We identified a total of 102 OM proteins, 52 are shared between the two red coral biominerals with scleritin being the most abundant protein in each fraction. Contrary to reef building corals, the red coral is collagen-rich (10 collagen-like proteins). Agrin-like glycoproteins and proteins with sugar-binding domains are also predominant. Twenty-seven and 23 proteins were uniquely assigned to the axial skeleton and the sclerites, respectively. Their inferred regulatory function suggests that the difference between the two biominerals rather relies on the modeling of the matrix network than on specific structural components. At least one OM component appears to have been horizontally transferred from prokaryotes early during Octocorallia evolution. Our results support the view that calcification of the red coral axial skeleton likely represents a secondary calcification of an ancestral gorgonian horny axis. In addition, the comparison with stony coral skeletomes highlighted the low proportion of similar proteins between the biomineral OMs of hexacorallian and octocorallian corals, suggesting an independent acquisition of calcification in anthozoans.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:08.791.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathalie Le Roy
SpeciesList	scientific name: Corallium rubrum; NCBI TaxID: 142104;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-13 03:33:19	ID requested
1	2022-02-15 13:14:15	announced
⏵ 2	2024-10-22 05:32:11	announced	2024-10-22: Updated project metadata.

Le Roy N, Ganot P, Aranda M, Allemand D, Tambutt, é S, The skeletome of the red coral Corallium rubrum indicates an independent evolution of biomineralization process in octocorals. BMC Ecol Evol, 21(1):1(2021) [pubmed]

10.1186/s12862-020-01734-0;

submitter keyword: sclerites, axial skeleton, organic matrix,Corallium rubrum, LC-MS/MS

Denis Allemand
contact affiliation	Centre scientifique de Monaco, Monaco
contact email	allemand@centrescientifique.mc
lab head
Nathalie Le Roy
contact affiliation	Bordeaux INP-ENSEGID
contact email	nathalie.le.roy5@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020332
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of Corallium rubrum biominerals