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PXD020323

PXD020323 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets
DescriptionPoly (ADP-ribose) polymerase 7 (PARP-7) has emerged as a critically important member of a large enzyme family that catalyze ADP-ribosylation in mammalian cells. PARP-7 is a critical regulator of the innate immune response. What remains unclear is the mechanism by which PARP-7 regulates this process, namely because the protein targets of PARP-7 mono-ADP-ribosylation (MARylation) are largely unknown. Here, we combine chemical genetics, proximity labeling, and proteome-wide amino acid ADP- ribosylation site profiling for identifying the direct targets and sites of PARP-7-mediated MARylation in a cellular context. We found that the inactive PARP family member, PARP13 (a critical regulator of the antiviral innate immune response) is a major target of PARP-7. PARP-13 is preferentially MARylated on cysteine residues in its RNA binding zinc finger domain. Proteome-wide ADP-ribosylation analysis reveals cysteine as a major MARylation acceptor of PARP-7. This study provides insight into PARP-7 targeting and MARylation site preference.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:24:08.571.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhillip Wilmarth
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetic acid derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-13 00:37:12ID requested
12021-07-21 15:06:32announced
22024-10-22 05:24:09announced2024-10-22: Updated project metadata.
Publication List
10.7554/elife.60480;
Rodriguez KM, Buch-Larsen SC, Kirby IT, Siordia IR, Hutin D, Rasmussen M, Grant DM, David LL, Matthews J, Nielsen ML, Cohen MS, Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets. Elife, 10():(2021) [pubmed]
Keyword List
submitter keyword: Chemical genetics, MARylation, PARP-7, protein-protein interactions, enzymatic targets
Contact List
Dr. Michael S. Cohen
contact affiliationDepartment of Chemical Physiology and Biochemistry Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239, USA
contact emailcohenmic@ohsu.edu
lab head
Phillip Wilmarth
contact affiliationOHSU
contact emailwilmarth@ohsu.edu
dataset submitter
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Dataset FTP location
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