PXD020320 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Target quantification of octapeptide for Thrombin and FX |
Description | Proteases are crucial physiologic regulators of protein structure and function. While proteomic methods contributed extensively to protease characterization efforts, technical challenges remain in terms of throughput, scalability and large datasets of protease cleavages remain scarce. Here, we describe a high-throughput protease screen (HTPS) which allows simultaneous characterization of multiple proteases under various conditions on a microscale 96FASP format. After benchmarking the performance with Trypsin, LysC, GluC, AspN, Chymotrypsin, MMP-2 and MMP-3, we applied it to profile proteases of the blood coagulation cascade (Factors VIIa, IXa, Xa, XIa, Thrombin α, β and γ, Plasmin and Protein C). The large dataset enabled us to map activity, specificity, cleave entropy, allosteric changes of blood cascade proteases induced by Na+ and to predict potential substrates in a data-driven way. Here, we designed two octapeptides, GIPRAAGD (α Thrombin) and GIGRRIAE (Factor Xa), and evaluated their cleavage with the target proteases. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-04 |
AnnouncementXML | Submission_2021-04-06_02:43:56.466.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Federico Uliana |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-13 00:19:18 | ID requested | |
1 | 2021-02-04 11:15:27 | announced | |
⏵ 2 | 2021-04-06 02:43:57 | announced | 2021-04-06: Updated publication reference for PubMed record(s): 33727531. |
Publication List
Uliana F, Vizovi, š, ek M, Acquasaliente L, Ciuffa R, Fossati A, Frommelt F, Goetze S, Wollscheid B, Gstaiger M, De Filippis V, Auf dem Keller U, Aebersold R, Mapping specificity, cleavage entropy, allosteric changes and substrates of blood proteases in a high-throughput screen. Nat Commun, 12(1):1693(2021) [pubmed] |
Keyword List
submitter keyword: blood coagulation proteases, specificity, entropy, allostery, substrates, high-throughput protease screen |
Contact List
Ruedi Aebersold |
contact affiliation | Department of Biology, Institute of Molecular Systems Biology, ETH Zürich, Zürich, Switzerland Faculty of Science, University of Zürich, Zürich, Switzerland |
contact email | aebersold@imsb.biol.ethz.ch |
lab head | |
Federico Uliana |
contact affiliation | ETH |
contact email | uliana@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020320
- Label: PRIDE project
- Name: Target quantification of octapeptide for Thrombin and FX