PXD020283

PXD020283 is an original dataset announced via ProteomeXchange.

Title	Evaluating the stress response in Candida albicans by data independent acquisition (DIA) proteomics
Description	Candida albicans is a fungal opportunistic pathogen responsible for cause severe infections in immunocompromised patients. Despite nowadays an effective antifungal armoury is available, the emergence of antifungal resistance is promoting the urge to develop alternative treatments to combat this fungal pathogen. For this purpose it is essential to go deepen in the knowledge of C. albicans response to several stressors. With this aim we performed a proteomic study of C. albicans exposed to 5 and 10 mM of hydrogen peroxide and 40 and 60 mM of acetic acid taking advantage of data independent acquisition mass spectrometry. Four biological replicates of each condition were analysed. For protein identification we used a C. albicans spectral library previously constructed by DDA analysis allowing the DIA quantitation of nearby 2000 proteins. Changes in protein abundance after the treatments reflected highly different proteomic patterns after each treatment. The exposure of C. albicans to hydrogen peroxide led to an increase in many proteins related to oxidative stress response, proteasome and protein folding while few proteins from mitochondria decreased their abundance. In contrast, acetic acid treatment triggered the decrease in the abundance of proteins related to aminoacid biosynthesis, actin polymerization, oxidative stress response and proteasome. This extensive proteomic study provided valuable information for the development of new drug targets against decisive proteins for cell survival.
HostingRepository	PRIDE
AnnounceDate	2021-09-13
AnnouncementXML	Submission_2022-02-21_03:03:50.444.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-09 17:22:01	ID requested
1	2021-09-13 08:37:23	announced
⏵ 2	2022-02-21 03:03:51	announced	2022-02-21: Updated publication reference for PubMed record(s): 34609160.

Amador-Garc, í, a A, Zapico I, Borrajo A, Malmstr, ö, m J, Monteoliva L, Gil C, Extending the Proteomic Characterization of Candida albicans Exposed to Stress and Apoptotic Inducers through Data-Independent Acquisition Mass Spectrometry. mSystems, 6(5):e0094621(2021) [pubmed]

submitter keyword: Candida albicans, DIA MS, oxidative stress, apoptosis, acetic acid, proteasome

Concha Gil García
contact affiliation	Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain
contact email	conchagil@ucm.es
lab head
Concha Gil
contact affiliation	UMC
contact email	cai.proteomica@pas.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020283

PRIDE project URI

• PRIDE
  1. PXD020283
     1. Label: PRIDE project
     2. Name: Evaluating the stress response in Candida albicans by data independent acquisition (DIA) proteomics