PXD020270

PXD020270 is an original dataset announced via ProteomeXchange.

Title	Identification of oxidized cysteine residues in signaling proteins in U937 cells at basal levels and upon diamide and hypochlorous acid stimulations.
Description	Here, we report a detection of oxidative post-translational modifications (ox-PTM) of Cys residues in the U937 cell line either non-stimulated or stimulated with diamide, a thiol-oxidizing agent at 0.5mM for 20min or HOCl at 100µM for 10min. Reversible Cys ox-PTMs were labelled using a bioswitch method using a Maleimide-(Polyethylene Glycol)2-Biotin (Mal-PEG2-Bio) probe independently of the oxidation type. Labeled proteins were analyzed by Mass spectrometry. We identified Cys ox-PTMs encompassing 1473 proteins containing at least ox-PTM-modified Cys. These sites include ox-PTM sites previously described in vitro validating the approach. Numerous novel Cys susceptible to ox-PTMs were identified including signaling proteins, including kinases, phosphatases and transcription and translation factors and proteins relevant to virus-host interactions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:10:32.033.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathalie Grandvaux
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	EDT-maleimide-PEO-biotin; N-ethylmaleimide derivatized cysteine; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-09 02:21:10	ID requested
1	2023-03-10 15:26:14	announced
⏵ 2	2023-11-14 08:10:32	announced	2023-11-14: Updated project metadata.

Cuervo NZ, Grandvaux N, Redox proteomics and structural analyses provide insightful implications for additional non-catalytic thiol-disulfide motifs in PDIs. Redox Biol, 59():102583(2023) [pubmed]

submitter keyword: post-translational modifications, mass spectrometry, oxidation,cysteine, redox, signaling

Nathalie Grandvaux
contact affiliation	CRCHUM-Centre Hospitalier de l'Université de Montréal, Montréal, QC H2X 0A9, Canada.
contact email	nathalie.grandvaux@umontreal.ca
lab head
Nathalie Grandvaux
contact affiliation	CRCHUM - Université de Montréal
contact email	labograndvaux@crchum.qc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD020270

PRIDE project URI

• PRIDE
  1. PXD020270
     1. Label: PRIDE project
     2. Name: Identification of oxidized cysteine residues in signaling proteins in U937 cells at basal levels and upon diamide and hypochlorous acid stimulations.