PXD020259

PXD020259 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of drought and high temperature response in roots of two potato cultivars
Description	The experiment was carried out during two vegetation seasons from 25th of April to 10th of September. Two Polish potato cultivars, the drought-tolerant Gwiazda and drought-sensitive Oberon were used. Selected tubers with transverse diameters of 3 - 4 cm were pre-sprouted for 2 weeks before planting. Plants were grown in a vegetation hall in pots filled with a thin layer of gravel on the bottom and the universal vegetable soil substrate ‘Hollas’ (Agaris Polska Ltd., Poland) produced from peat with the addition of chalk at a pH range of 5.5-6.5. Additionally, in phase 20 of the BBCH-scale of plant development, MIS-3 (Intermag) fertilizer was applied. Water content (WC) in volumetric basis in soil pots was measured according Black (1965). Weather conditions during the years of study were monitored using a Weather Campbell Station (Campbell Scientific Inc.) located in close proximity, and a thermohygrograph placed between pots. Meteorological data of air temperature, the photosynthetically active radiation, and humidity were comparable in the years of study and favorable for potato development. Three weeks after the initiation of the tuberisation phase (56 DAP), plants were divided into 3 groups, each consisting of 6 plants. The first group of plants was subjected to soil drought (remained without irrigation, day/night temperature 22°C/18°C), the second one to high temperature (day/night temperature 38°C/25°C) and the third one was watered according to needs and at optimal temperature (control plants, day/night temperature 22°C/18°C). Stress application lasted 14 days and finished at 70 DAP. During this period, plants were placed in phytotron equipped with six Hortilux Schreder Lamps with Philips light bulbs of 1600 W each. Air humidity was in the range 65-70%. WC under 14 days of soil drought reached 30% (v/v), and remained 80% (v/v) in control and high temperature conditions. During the recovery period, after 14 days of stress treatment, WC reached control levels. Plant root material for proteomic research was collected on the 14th day of stress treatment (70 DAP).
HostingRepository	PRIDE
AnnounceDate	2020-07-17
AnnouncementXML	Submission_2020-07-17_04:08:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020259
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marta Gietler
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-08 06:36:12	ID requested
⏵ 1	2020-07-17 04:08:08	announced

Dataset with its publication pending

submitter keyword: potato, Solanum tuberosum, Orbitrap

Małgorzata Nykiel
contact affiliation	Department of Biochemistry and Microbiology, Institute of Biology; Warsaw University of Life Sciences-SGGW
contact email	malgorzata_nykiel@sggw.edu.pl
lab head
Marta Gietler
contact affiliation	Department of Biochemistry and Microbiology, Institute of Biology, Warsaw University of Life Sciences‐SGGW, Warsaw, Poland
contact email	marta_gietler@sggw.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020259
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of drought and high temperature response in roots of two potato cultivars