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PXD020256

PXD020256 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThree protein extraction methods for mass spectrometry proteomics of fresh frozen mouse tissue
DescriptionWe present three different methods for protein extraction from fresh frozen mouse heart tissue: 1) extraction using SDS buffer followed by FASP purification, 2) extraction using SDS buffer followed by S-Trap purification, and 3) extraction using a guanidine hydrochloride buffer followed by in-solution digestion. Based on bicinchoninic acid assay, all three methods display similar recovery of total protein content. However, proteomics-based analysis identified far fewer proteins from the extraction guanidine hydrochloride. SDS-FASP identifies as many proteins as the SDS-STrap method with good coverage of proteins from different cellular compartments.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:10:00.653.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlex Kentsis
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-08 03:44:08ID requested
12020-07-29 22:00:02announced
22024-10-22 05:10:01announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mouse, LC-MS, Tissue, Proteomics
Contact List
Alex Kentsis
contact affiliationMolecular Pharmacology Program, Sloan Kettering Institute
contact emailkentsisresearchgroup@gmail.com
lab head
Alex Kentsis
contact affiliationMemorial Sloan Kettering Cancer Center
contact emailkentsisresearchgroup@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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