PXD020256 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Three protein extraction methods for mass spectrometry proteomics of fresh frozen mouse tissue |
Description | We present three different methods for protein extraction from fresh frozen mouse heart tissue: 1) extraction using SDS buffer followed by FASP purification, 2) extraction using SDS buffer followed by S-Trap purification, and 3) extraction using a guanidine hydrochloride buffer followed by in-solution digestion. Based on bicinchoninic acid assay, all three methods display similar recovery of total protein content. However, proteomics-based analysis identified far fewer proteins from the extraction guanidine hydrochloride. SDS-FASP identifies as many proteins as the SDS-STrap method with good coverage of proteins from different cellular compartments. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:10:00.653.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alex Kentsis |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-08 03:44:08 | ID requested | |
1 | 2020-07-29 22:00:02 | announced | |
⏵ 2 | 2024-10-22 05:10:01 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mouse, LC-MS, Tissue, Proteomics |
Contact List
Alex Kentsis |
contact affiliation | Molecular Pharmacology Program, Sloan Kettering Institute |
contact email | kentsisresearchgroup@gmail.com |
lab head | |
Alex Kentsis |
contact affiliation | Memorial Sloan Kettering Cancer Center |
contact email | kentsisresearchgroup@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020256
- Label: PRIDE project
- Name: Three protein extraction methods for mass spectrometry proteomics of fresh frozen mouse tissue