PXD020256

PXD020256 is an original dataset announced via ProteomeXchange.

Title	Three protein extraction methods for mass spectrometry proteomics of fresh frozen mouse tissue
Description	We present three different methods for protein extraction from fresh frozen mouse heart tissue: 1) extraction using SDS buffer followed by FASP purification, 2) extraction using SDS buffer followed by S-Trap purification, and 3) extraction using a guanidine hydrochloride buffer followed by in-solution digestion. Based on bicinchoninic acid assay, all three methods display similar recovery of total protein content. However, proteomics-based analysis identified far fewer proteins from the extraction guanidine hydrochloride. SDS-FASP identifies as many proteins as the SDS-STrap method with good coverage of proteins from different cellular compartments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:10:00.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Kentsis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-08 03:44:08	ID requested
1	2020-07-29 22:00:02	announced
⏵ 2	2024-10-22 05:10:01	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mouse, LC-MS, Tissue, Proteomics

Alex Kentsis
contact affiliation	Molecular Pharmacology Program, Sloan Kettering Institute
contact email	kentsisresearchgroup@gmail.com
lab head
Alex Kentsis
contact affiliation	Memorial Sloan Kettering Cancer Center
contact email	kentsisresearchgroup@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD020256

PRIDE project URI

• PRIDE
  1. PXD020256
     1. Label: PRIDE project
     2. Name: Three protein extraction methods for mass spectrometry proteomics of fresh frozen mouse tissue