PXD020254

PXD020254 is an original dataset announced via ProteomeXchange.

Title	Optimized Workflow for In-depth N-glycosylation Analysis of 2D and 3D Breast Cancer Cell Cultures and Xenografted Tumors
Description	Native intact N-glycopeptides analysis can access to a lot of useful information including N-glycosites, N-glycans and N-glycoproteins of original complex samples. The traditional sample preprocessing method is to analyze the tryptic intact N-glycopeptides enriched using hydrophilic materials or some new materials by LC-MS/MS. However, the number of identified intact N-glycopeptides was very limited using existing methods. Herein, we systematically compared the different analytical methods, including the use of different sources of trypsin, combinations of different proteases and different enrichment materials.
HostingRepository	PRIDE
AnnounceDate	2020-12-14
AnnouncementXML	Submission_2020-12-14_00:01:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-08 01:29:50	ID requested
⏵ 1	2020-12-14 00:01:41	announced

Mao Y, Zhao Y, Zhang Y, Yang H, In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors. PLoS One, 15(12):e0243789(2020) [pubmed]

submitter keyword: mass spectrometry, N-glycoproteomics, intact N-glycopeptides, zwitterionic hydrophilic interaction chromatography, breast cancer

Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
lab head
Yong Zhang
contact affiliation	Sichuan University
contact email	nankai1989@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD020254

PRIDE project URI

• PRIDE
  1. PXD020254
     1. Label: PRIDE project
     2. Name: Optimized Workflow for In-depth N-glycosylation Analysis of 2D and 3D Breast Cancer Cell Cultures and Xenografted Tumors