PXD020254 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Optimized Workflow for In-depth N-glycosylation Analysis of 2D and 3D Breast Cancer Cell Cultures and Xenografted Tumors |
Description | Native intact N-glycopeptides analysis can access to a lot of useful information including N-glycosites, N-glycans and N-glycoproteins of original complex samples. The traditional sample preprocessing method is to analyze the tryptic intact N-glycopeptides enriched using hydrophilic materials or some new materials by LC-MS/MS. However, the number of identified intact N-glycopeptides was very limited using existing methods. Herein, we systematically compared the different analytical methods, including the use of different sources of trypsin, combinations of different proteases and different enrichment materials. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-14 |
AnnouncementXML | Submission_2020-12-14_00:01:40.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yong Zhang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-08 01:29:50 | ID requested | |
⏵ 1 | 2020-12-14 00:01:41 | announced | |
Publication List
Mao Y, Zhao Y, Zhang Y, Yang H, In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors. PLoS One, 15(12):e0243789(2020) [pubmed] |
Keyword List
submitter keyword: mass spectrometry, N-glycoproteomics, intact N-glycopeptides, zwitterionic hydrophilic interaction chromatography, breast cancer |
Contact List
Yong Zhang |
contact affiliation | West China Hospital, Sichuan University |
contact email | nankai1989@foxmail.com |
lab head | |
Yong Zhang |
contact affiliation | Sichuan University |
contact email | nankai1989@foxmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD020254 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020254
- Label: PRIDE project
- Name: Optimized Workflow for In-depth N-glycosylation Analysis of 2D and 3D Breast Cancer Cell Cultures and Xenografted Tumors