PXD020248 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparison of LFQ and TMT-based quantitative proteomics towards toxicology research |
Description | Here, we aimed to compare two of the most commonly used global proteomics strategies, label-free quantification (LFQ) and tandem mass tag-based quantification (TMT) to identify the most suitable tool for toxicology research questions on the systemic level. Using a biological case study of HepG2 exposure to benzo[a]pyrene (BaP), we uncovered the advantages and limitations of the two strategies from the view of proteome coverage, fold changes, significant numbers of proteins, and pathway alterations. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:17:05.711.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhipeng Wang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-07 16:29:07 | ID requested | |
1 | 2021-03-04 12:57:58 | announced | |
⏵ 2 | 2024-10-22 05:17:06 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.tox.2020.152652; |
Wang Z, Karkossa I, Gro, ß, kopf H, Rolle-Kampczyk U, Hackerm, ü, ller J, von Bergen M, Schubert K, Comparison of quantitation methods in proteomics to define relevant toxicological information on AhR activation of HepG2 cells by BaP. Toxicology, 448():152652(2021) [pubmed] |
Keyword List
submitter keyword: toxicoproteomics, Benzo[a]pyrene, tandem mass tag (TMT), label free-quantification (LFQ), HepG2 |
Contact List
Kristin Schubert |
contact affiliation | Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research GmbH – UFZ, 04318 Leipzig, Germany |
contact email | kristin.schubert@ufz.de |
lab head | |
Zhipeng Wang |
contact affiliation | Helmholtz Centre for Environmental Research |
contact email | zhipeng.wang@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD020248 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020248
- Label: PRIDE project
- Name: Comparison of LFQ and TMT-based quantitative proteomics towards toxicology research