PXD020246

PXD020246 is an original dataset announced via ProteomeXchange.

Title	Correction of indels in the atpB gene by genetic recoding in the chloroplast of Oenothera and tobacco
Description	Genetic recoding, a process that modifies genome templated protein sequence, plays an important role especially in the small genomes of viruses and bacteria. Chloroplasts have evolved from cyanobacterial ancestry and mainly retain the prokaryotic gene expression machinery. I-iota, a plastome mutant of Oenothera (the evening primrose), carries a single adenine insertion in a polyA stretch near to the 5’-end of the atpB gene, encoding the β-CF1 subunit of the ATP synthase This causes formation of a truncated protein. Surprisingly, a full-length AtpB protein is detectable in I-iota leaves, suggesting the presence of genetic recoding operating in I-iota. In order to analyze this phenomenon in more detail, several tobacco transplastomic lines were generated inducing and destroying genetic recoding of atpB. We identified that a two adenine (+2A) insertion was more efficiently compensated in plants than +1A, -1A or -2A mutations at the same location. Furthermore, a homopolymeric composition of the polyA stretch is essential for successful recoding: plants carrying a destroyed polyA stretch have an albino-phenotype, indicating the absence of genetic recoding. Here we show evidence for genetic recoding acting in the chloroplast that plays presumablya compensatory function and enables correction of indels in an essential gene for autotrophic growth.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_13:11:33.297.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Witold Szymanski
SpeciesList	scientific name: Oenothera elata; NCBI TaxID: 44456;
ModificationList	iodoacetamide derivatized residue
Instrument	TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-07 16:28:27	ID requested
⏵ 1	2022-02-15 13:11:34	announced

Malinova I, Zupok A, Massouh A, Sch, ö, ttler MA, Meyer EH, Yaneva-Roder L, Szymanski W, R, ö, ß, ner M, Ruf S, Bock R, Greiner S, Correction of frameshift mutations in the atpB gene by translational recoding in chloroplasts of Oenothera and tobacco. Plant Cell, 33(5):1682-1705(2021) [pubmed]

submitter keyword: Oenothera, tabacco, SRM, proteomics, targeted, chloroplast

Dr. Search Results Web results Stephan Greiner
contact affiliation	Max-Planck-Institute of Molecular Plant Physiology
contact email	greiner@mpimp-golm.mpg.de
lab head
Witold Szymanski
contact affiliation	Max Planck Institute for Terrestrial Microbiology
contact email	szymanski@ie-freiburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020246
     1. Label: PRIDE project
     2. Name: Correction of indels in the atpB gene by genetic recoding in the chloroplast of Oenothera and tobacco