PXD020231

PXD020231 is an original dataset announced via ProteomeXchange.

Title	Protein interaction AP-MS data for TAP-CSAG1 and TAP-CSAG2 from HEK293 cells
Description	Ten 15 cm2 plates of HEK293 cells stably expressing TAP-vector, TAP-CSAG1, or TAP-CSAG2 were lysed with TAP lysis buffer (10% (v/v) glycerol, 50 mM HEPES-KOH pH 7.5, 100 mM KCl, 2 mM EDTA, 0.1% (v/v) NP-40, 10 mM NaF, 0.25 mM Na3VO4, 50 mM β-glyerolphosphate, 2 mM DTT, and 1X protease inhibitor cocktail (Sigma)) and cleared supernatants were bound to IgG-Sepharose beads (GE Amersham) and then washed in lysis buffer and TEV buffer (10 mM HEPES-KOH pH 8.0, 150 mM NaCl, 0.1% (v/v) NP-40, 0.5 mM EDTA, 1 mM DTT, and 1X protease inhibitor cocktail). Protein complexes were cleaved off the beads by TEV protease (Sigma) and incubated with calmodulin-Sepharose beads (GE Amersham) in calmodulin binding buffer (10 mM HEPES-KOH pH 8.0, 150 mM NaCl, 1 mM Mg acetate, 1 mM imidazole, 0.1% (v/v) NP-40, 6 mM CaCl2, 10 mM 2-mercaptoethanol) and then washed in calmodulin rinse buffer (50 mM Ammonium bicarbonate pH 8.0, 75 mM NaCl, 1 mM Mg Acetate, 1 mM Imidazole, 2 mM CaCl2) before eluted with SDS sample buffer, subjected to SDS-PAGE, and stained with GelCode Blue stain (Thermo Fisher Scientific) before protein identification by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:37.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrick Potts
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-07 04:34:36	ID requested
1	2020-08-31 22:08:52	announced
⏵ 2	2024-10-22 05:11:37	announced	2024-10-22: Updated project metadata.

10.15252/embr.202050912;

Yang X, Potts PR, CSAG2 is a cancer-specific activator of SIRT1. EMBO Rep, 21(9):e50912(2020) [pubmed]

submitter keyword: CSAG1 CSAG2

Patrick Ryan Potts
contact affiliation	Department of Cell and Molecular Biology St. Jude Children's Research Hospital Memphis, TN, 38105-3678, USA
contact email	ryan.potts@stjude.org
lab head
Patrick Potts
contact affiliation	St. Jude Children's Research Hospital
contact email	ryan.potts@stjude.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020231

PRIDE project URI

• PRIDE
  1. PXD020231
     1. Label: PRIDE project
     2. Name: Protein interaction AP-MS data for TAP-CSAG1 and TAP-CSAG2 from HEK293 cells