PXD020225 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Silica-cSilica-coated-magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate discovered using integrated omics analysisoated-magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate discovered using integrated omics analysis |
Description | Exposure to nanoparticles leads to their accumulation in the brain, but drug development to counteract this nanotoxicity remains challenging. Here we assessed the effect of silica-coated-magnetic nanoparticles containing the rhodamine B isothiocyanate dye [MNPs@SiO2(RITC)] on microglia through integration of transcriptomics, proteomics, and metabolomics. Intracellular reactive oxygen species production, an inflammatory response, and morphological activation of cells were greater, but glucose uptake was lower in MNPs@SiO2(RITC)-treated BV2 microglia and primary rat microglia. Expression of 121 genes, and levels of 45 proteins and 17 metabolites related to the above phenomena changed in MNPs@SiO2(RITC)-treated microglia. We integrated the three omics datasets and generated a single network using a machine learning algorithm. We screened 19 compounds and predicted their effects on nanotoxicity within the triple-omics network. A combination of glutathione and citrate attenuated nanotoxicity induced by MNPs@SiO2(RITC) and ten other nanoparticles in vitro and in the murine brain, protecting mostly the hippocampus and thalamus. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:32:11.681.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD020225 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ju Yeon Lee |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-06 15:12:08 | ID requested | |
1 | 2022-02-16 03:50:31 | announced | |
⏵ 2 | 2024-10-22 05:32:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.6019/PXD020225; |
10.1186/s12989-021-00433-y; |
Shin TH, Manavalan B, Lee DY, Basith S, Seo C, Paik MJ, Kim SW, Seo H, Lee JY, Kim JY, Kim AY, Chung JM, Baik EJ, Kang SH, Choi DK, Kang Y, Mouradian MM, Lee G, Silica-coated magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate identified using integrated omics. Part Fibre Toxicol, 18(1):42(2021) [pubmed] |
Keyword List
submitter keyword: machine learning,silica-coated- magnetic nanoparticles |
nanotoxicity |
integrated omics, microglia |
Contact List
Gwang Lee |
contact affiliation | Department of Physiology and Department of Biomedical Sciences, Ajou University School of Medicine, Suwon, Republic of Korea Immune-network pioneer research center, Ajou University School of Medicine, Suwon, Republic of Korea |
contact email | glee@ajou.ac.kr |
lab head | |
Ju Yeon Lee |
contact affiliation | Korea Basic Science Institute |
contact email | jylee@kbsi.re.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020225
- Label: PRIDE project
- Name: Silica-cSilica-coated-magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate discovered using integrated omics analysisoated-magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate discovered using integrated omics analysis