PXD020220 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of the dpp3 knockout mutant, the reconstituted strain and the wild-type strain of Candida lusitaniae |
Description | Candida lusitaniae is an emerging human opportunistic yeast, which can switch from yeast to pseudohyphae, and one of the rare Candida species capable of sexual reproduction. Its haploid genome and the genetic tools available make it a model of interest to study gene function. This study describes the consequences of DPP3 inactivation on cell morphology and mating, both altered in the dpp3Δ knock-out. Interestingly, reintroducing a wild-type copy of the DPP3 gene in the dpp3Δ mutant failed to restore the wild-type phenotypes. Proteomic analyses showed that about 150 proteins were statistically deregulated in the dpp3Δ mutant, and that most of them did not return to their wild-type level in the reconstituted DPP3 strain. The analysis of the segregation of the dpp3Δ mutation and the phenotypes in the progeny of a cross (between the dpp3Δ knock-out and a wild-type strain) showed that the phenotypes are not linked to dpp3Δ, but to a secondary mutation. Genome sequencing of the dpp3Δ mutant allowed us to identify this secondary mutation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:45:19.945.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dupuy Jean-William |
SpeciesList | scientific name: Clavispora lusitaniae; NCBI TaxID: 36911; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-06 06:03:40 | ID requested | |
1 | 2023-05-02 02:46:08 | announced | |
⏵ 2 | 2023-11-14 08:45:21 | announced | 2023-11-14: Updated project metadata. |
Publication List
Sabra A, Biteau N, Dupuy JW, Klopp C, No, ë, l T, Dementhon K, . J Fungi (Basel), 9(3):(2023) [pubmed] |
Keyword List
submitter keyword: secondary mutation, quantitative proteome,Candida, mating, cell separation, pseudo-hyphal growth |
Contact List
Karine Dementhon |
contact affiliation | Microbiologie Fondamentale et Pathogénicité, UMR 5234 (Univ. Bordeaux, CNRS), Bordeaux, France. |
contact email | karine.dementhon@u-bordeaux.fr |
lab head | |
Dupuy Jean-William |
contact affiliation | Plateforme Proteome Bordeaux |
contact email | jean-william.dupuy@u-bordeaux.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020220
- Label: PRIDE project
- Name: Proteomic analysis of the dpp3 knockout mutant, the reconstituted strain and the wild-type strain of Candida lusitaniae