PXD020219

PXD020219 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of extracellular vesicles and conditioned medium from human Adipose-derived Stem/Stromal Cells and Dermal Fibroblasts
Description	Conditioned medium (CM) and extracellular vesicles (EV) from human Adipose-derived Stem/stromal cells (ASC) and Dermal fibroblasts (DF) can represent valid cell substitutes in regenerative medicine and other therapeutic applications. Whether one of the two cell products should be preferred over the other is still under debate and no direct comparison of their proteome has been reported yet. Here, we apply a comprehensive quantitative proteomics approach to explore the protein composition of conditioned medium and EV obtained from the two cell types. We identified 1977 proteins by LC-MS/MS proteomic analysis. Unsupervised clustering analysis and PCA clearly distinguished CM and EV as separate groups, regardless the cell source. We identified 68 and 201 proteins that were more abundant or exclusively detectable in CM and EV, respectively. CM were enriched in proteins of endoplasmic reticulum, Golgi apparatus and lysosomes, whereas EV contained a large amount of GTPases, ribosome and translation-related factors. Moreover, the analysis of ASC and DF secretomes (both CM and EV) revealed the presence of cell type-specific factors. ASC-CM and -EV carried factors involved in ECM organization (hyaluronan and glycosaminoglycan metabolism) and immunological regulation (e.g. macrophage and IkB/NFkB signaling regulation), respectively. On the other hand, DF-CM and –EV were both enriched in epithelium development associated factors, whilst DF-CM in proteins involved in cellular processes regulation and -EV in Wnt signaling factors. In conclusion, this comprehensive proteomic analysis of the secretome components of ASC and DF, provides evidence of a different protein composition between CM and EV and of the presence of cell type-specific bioactive mediators suggesting their specific future use as advanced therapy medicinal products.
HostingRepository	PRIDE
AnnounceDate	2020-12-23
AnnouncementXML	Submission_2020-12-23_01:59:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020219
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Annapaola Andolfo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 05:01:58	ID requested
⏵ 1	2020-12-23 01:59:03	announced

Niada S, Giannasi C, Magagnotti C, Andolfo A, Brini AT, Proteomic analysis of extracellular vesicles and conditioned medium from human adipose-derived stem/stromal cells and dermal fibroblasts. J Proteomics, 232():104069(2021) [pubmed]

submitter keyword: Label-free quantitative proteomics, secretome, conditioned medium, extracellular vesicles, adipose-derived stromal cells, dermal fibroblasts

Anna Teresa Brini
contact affiliation	IRCCS Istituto Ortopedico Galeazzi, Laboratorio di Applicazioni Biotecnologiche, Milan, Italy Department of Biomedical, Surgical and DentalSciences, Università degli Studi di Milano, Milan, Italy
contact email	anna.brini@unimi.it
lab head
Annapaola Andolfo
contact affiliation	Ospedale San Raffaele
contact email	andolfo.annapaola@hsr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020219
     1. Label: PRIDE project
     2. Name: Proteomic analysis of extracellular vesicles and conditioned medium from human Adipose-derived Stem/Stromal Cells and Dermal Fibroblasts