PXD020217

PXD020217 is an original dataset announced via ProteomeXchange.

Title	Human Tear using MALDI-TOF-MS for Dry Eye syndrome
Description	Tears are a biological fluid that has diagnostic potential for ocular diseases. Extracellular vesicles (EVs), wildly detected in various biofluids including tears, are nanoparticles released by living cells and considered as promising detection sources for non-invasive liquid biopsy. Understanding the roles of tears and tear-EVs in ocular diseases such as dry eye can facilitate the studies of clinical diagnosis, which usually entails detecting such liquid objects with a rapid and effective method. In this study, we utilized a mass spectrometry based strategy to analyze peptidome/proteome profiles of tear and EVs for rapid dry eye diagnosis. Nano-sized EVs were isolated from tears of either healthy control (HC) individuals or dry eye syndrome (DES) patients, and the tear compositions were further analyzed by tracking their fingerprints with matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). The fingerprints of tear-EVs could be observed in a dose-dependent manner as well as tears, allowing comparing their discriminant peaks between tears and EVs. By analyzing these peaks, the fingerprints of both tear and tear-EVs were showed to have a capability of distinguishing DES patients from HC donors, and providing an efficient way for screening potential DES biomarkers. The proposed tear and EV fingerprinting approach is expected to be a potential tool in rapid diagnosis of ocular disease and in-depth researches of pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2020-08-20
AnnouncementXML	Submission_2020-08-20_03:38:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 02:37:19	ID requested
⏵ 1	2020-08-20 03:38:34	announced

Zhang T, Hu L, Ma H, Ni F, Liu F, Chen H, Detection of Tear Components Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry for Rapid Dry Eye Diagnosis. J Proteome Res, 19(9):3644-3651(2020) [pubmed]

submitter keyword: Human, Tear, Extracellular vesicles, MALDI-TOF MS, dry eye syndrome

Fei Liu
contact affiliation	Wenzhou Medical University
contact email	feiliu@wmu.edu.cn
lab head
Ting Zhang
contact affiliation	Wenzhou Medical University
contact email	ting6240@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD020217

PRIDE project URI

• PRIDE
  1. PXD020217
     1. Label: PRIDE project
     2. Name: Human Tear using MALDI-TOF-MS for Dry Eye syndrome