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PXD020215

PXD020215 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDevelopment of a multiplexed platform to study laccases and cellulase activities using nanostructure-initiator mass spectrometry (NIMS)
DescriptionLignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin. Although lignin has long been considered a waste product in biomass conversion efforts, its utilization has since been identified as critical to the economic viability of second-generation biofuel production. There is thus increasing interest in finding enzymes and enzyme cocktails which can efficiently deconstruct both the cellulose/hemicellulose and lignin components of lignocellulosic biomass. Analytical tools capable of quickly detecting both glycan and lignin deconstruction could are needed to support the development and characterization of efficient enzymes/enzyme cocktails.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:28:15.961.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD020215
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Petzold
SpeciesList scientific name: Aspergillus flavus var. oryzae; NCBI TaxID: 748371; scientific name: Agaricus bisporus var. bisporus; NCBI TaxID: 192523;
ModificationListNo PTMs are included in the dataset
Instrument6550 iFunnel Q-TOF LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-06 02:11:39ID requested
12021-09-09 01:12:34announced
22024-10-22 05:28:18announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD020215;
10.1038/s41598-021-91181-8;
Ing N, Deng K, Chen Y, Aulitto M, Gin JW, Pham TLM, Petzold CJ, Singer SW, Bowen B, Sale KL, Simmons BA, Singh AK, Adams PD, Northen TR, A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities. Sci Rep, 11(1):11803(2021) [pubmed]
Keyword List
submitter keyword: Laccases, Multiplex assay, Lignin, NIMS,GHs (glycosyl hydrolases), Enzyme assays
Contact List
Christopher J. Petzold
contact affiliationStaff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact emailcjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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