PXD020215

PXD020215 is an original dataset announced via ProteomeXchange.

Title	Development of a multiplexed platform to study laccases and cellulase activities using nanostructure-initiator mass spectrometry (NIMS)
Description	Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin. Although lignin has long been considered a waste product in biomass conversion efforts, its utilization has since been identified as critical to the economic viability of second-generation biofuel production. There is thus increasing interest in finding enzymes and enzyme cocktails which can efficiently deconstruct both the cellulose/hemicellulose and lignin components of lignocellulosic biomass. Analytical tools capable of quickly detecting both glycan and lignin deconstruction could are needed to support the development and characterization of efficient enzymes/enzyme cocktails.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:15.961.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020215
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Aspergillus flavus var. oryzae; NCBI TaxID: 748371; scientific name: Agaricus bisporus var. bisporus; NCBI TaxID: 192523;
ModificationList	No PTMs are included in the dataset
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 02:11:39	ID requested
1	2021-09-09 01:12:34	announced
⏵ 2	2024-10-22 05:28:18	announced	2024-10-22: Updated project metadata.

10.6019/PXD020215;

10.1038/s41598-021-91181-8;

Ing N, Deng K, Chen Y, Aulitto M, Gin JW, Pham TLM, Petzold CJ, Singer SW, Bowen B, Sale KL, Simmons BA, Singh AK, Adams PD, Northen TR, A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities. Sci Rep, 11(1):11803(2021) [pubmed]

submitter keyword: Laccases, Multiplex assay, Lignin, NIMS,GHs (glycosyl hydrolases), Enzyme assays

Christopher J. Petzold
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020215

PRIDE project URI

• PRIDE
  1. PXD020215
     1. Label: PRIDE project
     2. Name: Development of a multiplexed platform to study laccases and cellulase activities using nanostructure-initiator mass spectrometry (NIMS)