PXD020215 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Development of a multiplexed platform to study laccases and cellulase activities using nanostructure-initiator mass spectrometry (NIMS) |
Description | Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin. Although lignin has long been considered a waste product in biomass conversion efforts, its utilization has since been identified as critical to the economic viability of second-generation biofuel production. There is thus increasing interest in finding enzymes and enzyme cocktails which can efficiently deconstruct both the cellulose/hemicellulose and lignin components of lignocellulosic biomass. Analytical tools capable of quickly detecting both glycan and lignin deconstruction could are needed to support the development and characterization of efficient enzymes/enzyme cocktails. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:28:15.961.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD020215 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Christopher Petzold |
SpeciesList | scientific name: Aspergillus flavus var. oryzae; NCBI TaxID: 748371; scientific name: Agaricus bisporus var. bisporus; NCBI TaxID: 192523; |
ModificationList | No PTMs are included in the dataset |
Instrument | 6550 iFunnel Q-TOF LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-06 02:11:39 | ID requested | |
1 | 2021-09-09 01:12:34 | announced | |
⏵ 2 | 2024-10-22 05:28:18 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.6019/PXD020215; |
10.1038/s41598-021-91181-8; |
Ing N, Deng K, Chen Y, Aulitto M, Gin JW, Pham TLM, Petzold CJ, Singer SW, Bowen B, Sale KL, Simmons BA, Singh AK, Adams PD, Northen TR, A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities. Sci Rep, 11(1):11803(2021) [pubmed] |
Keyword List
submitter keyword: Laccases, Multiplex assay, Lignin, NIMS,GHs (glycosyl hydrolases), Enzyme assays |
Contact List
Christopher J. Petzold |
contact affiliation | Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720 |
contact email | cjpetzold@lbl.gov |
lab head | |
Christopher Petzold |
contact affiliation | Lawrence Berkeley National Laboratory |
contact email | cjpetzold@lbl.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020215
- Label: PRIDE project
- Name: Development of a multiplexed platform to study laccases and cellulase activities using nanostructure-initiator mass spectrometry (NIMS)