PXD020213

PXD020213 is an original dataset announced via ProteomeXchange.

Title	Identification of GltB-interacting proteins by CoIP-MS
Description	To investigate whether GltB possess more function, Co-immunoprecipitation coupled with Mass Spectrometry (CoIP-MS) was used to isolate the putative GltB binding proteins.We used strains PAO1/p-gtrS-YFP and ΔgltB/p-gtrS-YFP to ensure the membrane protein GtrS of great interest is at high levels. A target GltB-specific antibody anti-GltB was used and ΔgltB/p-gtrS-YFP was set up as a negative control. The whole gel lane was divided into small fractions and in-gel digested for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Control sample was also analyzed in parallel to distinguish the background proteins.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_03:56:34.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chenchen Xu
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 00:57:31	ID requested
⏵ 1	2021-09-09 03:56:34	announced

Xu C, Cao Q, Lan L, . Microorganisms, 9(2):(2021) [pubmed]

submitter keyword: pseudomonas aeruginosa,GltB-interacting protein,LC-MS/MS

Lefu Lan
contact affiliation	Shanghai institute of materia medica
contact email	llan@simm.ac.cn
lab head
Chenchen Xu
contact affiliation	Shanghai Institute of Materia Medica Chinese Academy of Sciences
contact email	201528012342123@simm.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020213
     1. Label: PRIDE project
     2. Name: Identification of GltB-interacting proteins by CoIP-MS