PXD020195

PXD020195 is an original dataset announced via ProteomeXchange.

Title	Creation of a Candida albicans spectral library by Data Dependent Acquisition (DDA)
Description	The incidence of candidiasis, caused mainly by the opportunistic fungal Candida albicans, remains a major concern in public health due to the emerging antifungal resistance. C. albicans modulates their proteome under different conditions trying to cope with several stressors in host niches. In this study we developed a C. albicans spectral library by analysing a total of 52 samples corresponding to cells treated with 5 and 10 mM hydrogen peroxide, 40 and 60 mM acetic acid and in the interaction with human macrophages (THP-1) at 3, 6 and 9 h (4 biological replicates). This samples represented several morphologies of C. albicans (yeast and hypha) and different culture conditions (temperature, media…) that contributed to the incorporation of different proteins into the library. The analysis of these conditions by DDA mode allowed the construction of a C. albicans spectral library comprising information of 2879 proteins and 25645 peptides which entailed a 46.5% of the total proteome. The C. albicans spectral library created constitutes a powerful proteomic tool available for the scientific community and could be useful for global (DIA) or targeted (SRM and PRM) studies.
HostingRepository	PRIDE
AnnounceDate	2021-09-30
AnnouncementXML	Submission_2022-02-16_03:23:01.874.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduardo Romeu
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-05 23:03:23	ID requested
1	2021-09-30 09:39:15	announced
⏵ 2	2022-02-16 03:23:02	announced	2022-02-16: Updated publication reference for PubMed record(s): 34609160.

Amador-Garc, í, a A, Zapico I, Borrajo A, Malmstr, ö, m J, Monteoliva L, Gil C, Extending the Proteomic Characterization of Candida albicans Exposed to Stress and Apoptotic Inducers through Data-Independent Acquisition Mass Spectrometry. mSystems, 6(5):e0094621(2021) [pubmed]

submitter keyword: Candida albicans, DDA MS, spectral library, DIA MS, hydrogen peroxide, acetic acid, macrophages

Concha Gil García
contact affiliation	Concha Gil, Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain
contact email	conchagil@ucm.es
lab head
Eduardo Romeu
contact affiliation	UCM
contact email	eromeu@ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020195

PRIDE project URI

• PRIDE
  1. PXD020195
     1. Label: PRIDE project
     2. Name: Creation of a Candida albicans spectral library by Data Dependent Acquisition (DDA)