PXD020190

PXD020190 is an original dataset announced via ProteomeXchange.

Title	The trypanosome UDP-glucose pyrophosphorylase is active in both the glycosomal and cytosolic compartments and is imported into glycosomes by piggybacking
Description	Trypanosoma brucei, the etiological agent of sleeping sickness, has particular peroxisome-like organelles called glycosomes where several metabolic pathways are compartmentalized, including glycolysis. Many enzymes from sugar nucleotide biosynthetic pathways are located in this organelle, contrasting with the cytosolic localisation reported in the eukaryota domain. The UDP-glucose pyrophosphorylase (UGP), the enzyme responsible for UDP-glucose (UDP-Glc) production, is essential for growth and survival of trypanosomes. In addition, UGP is localized in both glycosomes and cytosol, raising questions about why would these parasites maintain a pathway functional in two different subcellular compartments. UGP is imported into glycosomes by piggybacking on the glycosomal protein PEPCK, which is the first evidence of piggybacking involving two proteins not functionally related, and the first report in trypanosomatids. The dataset demonstrates that UGP is present in the total cellular extract and the glycocomal fraction of the wild-type trypanosomes, while only in the total cellular extract of the PEPCK null mutant.
HostingRepository	PRIDE
AnnounceDate	2021-06-10
AnnouncementXML	Submission_2021-06-10_08:14:53.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-03 08:52:13	ID requested
⏵ 1	2021-06-10 08:14:54	announced

Villafraz O, Baudouin H, Mazet M, Kulyk H, Dupuy JW, Pineda E, Bott, é C, Inaoka DK, Portais JC, Bringaud F, The Trypanosome UDP-Glucose Pyrophosphorylase Is Imported by Piggybacking into Glycosomes, Where Unconventional Sugar Nucleotide Synthesis Takes Place. mBio, 12(3):e0037521(2021) [pubmed]

submitter keyword: Trypanosoma brucei, label-free quantitative proteome analysis

Frédéric Bringaud
contact affiliation	Laboratoire de Microbiologie Fondamentale et Pathogénicité (MFP), Université de Bordeaux, CNRS UMR-5234, Bordeaux, France
contact email	frederic.bringaud@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020190
     1. Label: PRIDE project
     2. Name: The trypanosome UDP-glucose pyrophosphorylase is active in both the glycosomal and cytosolic compartments and is imported into glycosomes by piggybacking