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PXD020189

PXD020189 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Protein Kinase G-interacting protein regulates critical calcium signals in malaria parasites (phosphoproteomic)
DescriptionIn malaria parasites, cGMP signalling is mediated by a single cGMP-dependent protein kinase (PKG). One of the major functions of PKG is to control calcium signals essential for the parasite to exit red blood cells or for the transmission of the gametocyte stages to the mosquito. However, how PKG controls these signals in the absence of known second messenger-dependent calcium channels or scaffolding proteins remains a mystery. Here we use pull-down approaches to identify a PKG partner protein in both Plasmodium falciparum schizonts and P. berghei gametocytes. This partner, named ICM1, is a polytopic membrane protein with homologies to transporters and calcium channels, raising the possibility of a direct functional link between PKG and calcium homeostasis. Phosphoproteomic analyses in both Plasmodium species highlight a densely phosphorylated region of ICM1 with multiple phosphorylation events dependent on PKG activity. Conditional disruption of the P. falciparum ICM1 gene results in reduced cGMP-dependent calcium mobilisation associated with defective egress and invasion. Stage-specific depletion of ICM1 in P. berghei gametocytes blocks gametogenesis due to the inability of mutant parasites to mobilise intracellular calcium upon PKG activation. These results provide us with new insights into the atypical calcium homeostasis in malaria parasites
HostingRepositoryPRIDE
AnnounceDate2021-01-25
AnnouncementXMLSubmission_2021-01-24_22:32:21.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD020189
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitteralexandre Hainard
SpeciesList scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-03 08:52:12ID requested
12021-01-24 22:32:21announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: plasmodium, gametocytes, Phosphoproteomic, LC-MSMS
Contact List
Mathieu Brochet
contact affiliationDepartment of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, CH-1211 Switzerland
contact emailmathieu.brochet@unige.ch
lab head
alexandre Hainard
contact affiliationUniversity of Geneva
contact emailalexandre.hainard@unige.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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