PXD020185

PXD020185 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Metabolic selection of a homologous recombination mediated gene rearrangement in Trypanosoma brucei
Description	The Trypanosoma brucei genome contains three fumarate reductase (FRD) genes, including two tandemly arranged in chromosome 5 encoding the glycosomal isoform (FRDg) and a potentially mitochondrial FRD isoform never detected so far in trypanosomes (FRDm2). We report here that the PPDK/PEPCK/GPDH mutant cell line (Δppdk/Δpepck/RNAi-GPDH) expresses a chimeric non-functional FRDg-m2 isoform resulting from stochastic homologous recombination within the FRDg/FRDm2 locus. This conclusion come from comparison of the parental (WT, Δppdk, Δpepck and Δppdk/Δpepck) and Δppdk/Δpepck/RNAi-GPDH proteomes. The selective advantage provided by the consequential loss of the FRDg gene, in the context of the Δppdk/Δpepck/RNAi-GPDH null background, is probably related to the autoflavinylation activity of the FRDg isoform.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:22:52.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-03 07:21:31	ID requested
1	2021-06-10 08:19:24	announced
⏵ 2	2024-10-22 05:22:58	announced	2024-10-22: Updated project metadata.

Publication List

10.1016/j.jbc.2021.100548;
Wargnies M, Plazolles N, Schenk R, Villafraz O, Dupuy JW, Biran M, Bachmaier S, Baudouin H, Clayton C, Boshart M, Bringaud F, Metabolic selection of a homologous recombination-mediated gene loss protects Trypanosoma brucei from ROS production by glycosomal fumarate reductase. J Biol Chem, 296():100548(2021) [pubmed]

10.1016/j.jbc.2021.100548;

Wargnies M, Plazolles N, Schenk R, Villafraz O, Dupuy JW, Biran M, Bachmaier S, Baudouin H, Clayton C, Boshart M, Bringaud F, Metabolic selection of a homologous recombination-mediated gene loss protects Trypanosoma brucei from ROS production by glycosomal fumarate reductase. J Biol Chem, 296():100548(2021) [pubmed]

Keyword List

submitter keyword: Trypanosoma brucei, label-free quantitative proteome analysis

submitter keyword: Trypanosoma brucei, label-free quantitative proteome analysis

Contact List

Frédéric Bringaud
contact affiliation	Laboratoire de Microbiologie Fondamentale et Pathogénicité (MFP), Université de Bordeaux, CNRS UMR-5234, Bordeaux, France
contact email	frederic.bringaud@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD020185
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD020185

PRIDE project URI

Repository Record List

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