PXD020167

PXD020167 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry of P. tricornutum proteome, Bohlin clone Pt1 8.6 (CCMP632) culture, cpsrp54-8, cpsrp54-11.
Description	The chloroplast signal recognition particle 54 kDa (CpSRP54) is a member of the CpSRP pathway targeting proteins to thylakoid membranes. In plants, CpSRP54 has a dual function and is involved in both co-translational and post-translational insertion of thylakoid membrane proteins like subunits of photosynthetic complexes and light harvesting antenna proteins, respectively. In green microalgae, CpSRP54 seems to function only in the post-translational part of the CpSRP pathway. The effects of loss of CpSRP54 in the diatom model species Phaeodactylum tricornutum support a role in co-translational targeting of proteins to thylakoid membranes, but not in the post-translational CpSRP pathway. Lack of CpSRP54 does not have a negative effect on the content of light harvesting antenna proteins and pigments, but causes lower levels of a selection of PSII, PSI, Cytb6f and ATP synthase subunits. Cpsrp54 lines also display decreased photophysiological fitness, stronger induction of photoprotective mechanisms and lower growth rates compared to wild type when exposed to increased light intensities. Mechanisms alleviating the loss of CpSRP54, involving upregulation of chaperones, might explain the still relatively mild phenotype of the cpsrp54 mutants.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:19:58.524.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020167
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Phaeodactylum tricornutum (strain CCAP 1055/1); NCBI TaxID: 556484;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-02 18:34:07	ID requested
1	2021-03-12 01:52:34	announced
2	2021-03-12 04:01:42	announced	2021-03-12: Updated publication reference for PubMed record(s): 33372269.
⏵ 3	2024-10-22 05:20:00	announced	2024-10-22: Updated project metadata.

10.1111/tpj.15149;

Nymark M, Gr, ø, nbech Hafskjold MC, Volpe C, Fonseca DM, Sharma A, Tsirvouli E, Serif M, Winge P, Finazzi G, Bones AM, Functional studies of CpSRP54 in diatoms show that the mechanism of thylakoid protein insertion differs from that in plants and green algae. Plant J, 106(1):113-132(2021) [pubmed]

submitter keyword: CRISPR, Cas9

Atle Bones
contact affiliation	Professor, Department of Biology, Norwegian University of Science and Technology, N-7491, Trondheim, Norway
contact email	atle.m.bones@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD020167

PRIDE project URI

• PRIDE
  1. PXD020167
     1. Label: PRIDE project
     2. Name: Mass spectrometry of P. tricornutum proteome, Bohlin clone Pt1 8.6 (CCMP632) culture, cpsrp54-8, cpsrp54-11.