PXD020156

PXD020156 is an original dataset announced via ProteomeXchange.

Title	Analysis of Tim-3 interactome in primary mouse CD4+ T cells
Description	We analyzed the interactome of the Tim-3 (HAVCR2) protein, using an affinity purification method based on the the endogenous expression of a One-Strep-tagged (OST) version of Tim-3 in an engineered mouse model, and affinity purification of the protein from expanded primary CD4+ T cells. Affinity purification of the OST tagged protein was performed using Streptactin beads, from T cells either non stimulated, or stimulated 30s, 120s, 300s or 900s with pervanadate. Each AP-MS purification is associated with a corresponding control (purification from CD4+ T cells isolated from WT mice, cultured and stimulated in the same conditions). The number of replicate biological experiments was n=3 for all conditions (time-points), and each sample was analyzed twice by nanoLC-MS, resulting in 60 raw files.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:38:00.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-02 15:30:57	ID requested
1	2022-06-30 05:36:07	announced
⏵ 2	2024-10-22 05:38:01	announced	2024-10-22: Updated project metadata.

Zhai Y, Celis-Gutierrez J, Voisinne G, Mori D, Girard L, Burlet-Schiltz O, de Peredo AG, Roncagalli R, Malissen B, Opposing regulatory functions of the TIM3 (HAVCR2) signalosome in primary effector T cells as revealed by quantitative interactomics. Cell Mol Immunol, 18(6):1581-1583(2021) [pubmed]

10.1038/s41423-020-00575-7;

submitter keyword: AP-MS,TCR, primary CD4+ T cell, interactome, signalosome

Odile Schiltz
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse
contact email	odile.schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD020156

PRIDE project URI

• PRIDE
  1. PXD020156
     1. Label: PRIDE project
     2. Name: Analysis of Tim-3 interactome in primary mouse CD4+ T cells