PXD020152

PXD020152 is an original dataset announced via ProteomeXchange.

Title	Proteome of gamma-butyrobetaine-grown Eubacterium limosum ATCC 8486
Description	Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of gamma-butyrobetaine -grown Eubacterium limosum was obtained in order to identify enzymes required for growth on gamma-butyrobetaine, in particular to identify components that are unique to growth on gamma-butyrobetaine in comparison to other substrates for acetogenesis, such as lactic acid, L-carnitine, or proline betaine. Gamma-butyrobetaine is converted to trimethylamine (TMA) by certain members of the gut microbiome. Subsequent liver metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of gamma-butyrobetaine is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Gamma-butyrobetaine demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherosclerosis caused by metabolism of TMA in the body. This proteome led to discovery of gamma-butyrobetaine:tetrahydrofolate methyltransferase system. The key gamma-butyrobetaine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:15.451.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020152
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joseph Krzycki
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Eubacterium limosum; NCBI TaxID: 1736;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-02 02:26:13	ID requested
1	2021-11-04 09:51:04	announced
⏵ 2	2024-10-22 05:30:15	announced	2024-10-22: Updated project metadata.

10.6019/PXD020152;

submitter keyword: Eubacterium limosum, gut microbiome, butyrobetaine, acetogen

Joseph A. Krzycki
contact affiliation	Department of Microbiology, The Ohio State University, Columbus OH
contact email	Krzycki.1@osu.edu
lab head
Joseph Krzycki
contact affiliation	The Ohio State University
contact email	Krzycki.1@osu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020152
     1. Label: PRIDE project
     2. Name: Proteome of gamma-butyrobetaine-grown Eubacterium limosum ATCC 8486