PXD020145

PXD020145 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic characterization of human bile reveals the interplay between host and the microbiota
Description	Cholesterol gallstone is a common disease, but the pathogenesis of gallstone remains a mystery. The pathogenetic factors of gallstone disease include a genetic background, cholesterol condensation and bile supersaturation, while the risk factors may include fatty liver, diabetes, obesity and microbiome dysbiosis. Dysbiosis refers to altered bacterial composition and abundance, which is found to be associated with the pathogenesis of variety of diseases. Mass spectrometry-based proteomics emerges as a powerful tool to identify protein expressions of human samples. A number of proteomic studies have been performed to analyze human protein contents of bile samples. All these studies focus on the analysis of human proteins, and no study to date has been performed to analyze the protein expression of microbiome in bile samples associated with gallstone disease. In current study, we performed a label-free metaproteomic analysis of gallbladder bile samples from cholesterol gallstone disease patients and normal individuals. ProteoStorm was used for the first-step comprehensive database searching to identify microbial and host peptides, and Maxquant was used in the second-step refined database searching to infer protein groups with quantitation. We attempt to investigate the dysbiosis changes, potential virulence factor of microbiome and host immune responses which contribute to gallstone formation and cholelithiasis.
HostingRepository	iProX
AnnounceDate	2020-07-02
AnnouncementXML	Submission_2020-07-01_21:12:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-01 21:10:42	ID requested
⏵ 1	2020-07-01 21:12:53	announced

Dataset with its publication pending

submitter keyword: Bile, Metaproteomics, Gallstone, microbiome, Bacteria, Proteomics, Cholecystitis

Feng Liu
contact affiliation	Institutes of Biomedical of Sciences, Fudan University
contact email	liuf@fudan.edu.cn
lab head
Jie Wang
contact affiliation	Institutes of Biomedical of Sciences, Fudan University
contact email	tlwj1949@163.com
dataset submitter

iProX dataset URI